FastQCFastQC Report
Mon 3 Jul 2023
SRR5892473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5892473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151164969
Total Bases15.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT54777193.623669581806351No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT51907373.433822686789292No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAATA29987531.9837618595350621No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGGCTGTAGGCACCATCAATAGAT22418511.4830492903418648No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAA20762071.3734709924757766No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAAT19537551.2924654520982304No Hit
TCCGGTCCTATTGTGTTGGCCTTCGGGATCGGACTGTAGGCACCATCAAT13460710.8904649065882453No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAATA10907470.7215606943960674No Hit
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA9969170.65948943501586No Hit
CCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT8130490.5378554339530873No Hit
TTGGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCA7590640.5021427947370531No Hit
TACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCAATAG5410430.35791559617228513No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA5406530.35765759989009094No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAAT5106830.33783157789686047No Hit
CCGAGAGACCCGGGTTCGTGTCCCGGCAACGGACTGTAGGCACCATCAAT4944300.3270797482186498No Hit
TCGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGG4871730.3222790327830517No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATA4787000.3166738981701508No Hit
ACCGTCCACGCCCGGTCGTACTCATAACCGCACTGTAGGCACCATCAATA4756990.31468864985511297No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAA4474350.2959911962142499No Hit
TCCTGCGGCAAAATAGCTCGATGCCAGAATCTGTAGGCACCATCAATAGA4372560.28925749324898153No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT4272050.2826084659865872No Hit
CCGAGAGACCCGGGTTCGGGTCCCGGCAACGGACTGTAGGCACCATCAAT4269280.2824252224733364No Hit
TCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT4188920.2771091760022787No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG4023760.2661833642158191No Hit
ACTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAAT3845330.2543797035409705No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGCTGTAGGCACCATCAATA3775970.24979133889148616No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATA3697470.24459833680116722No Hit
TTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGACTGTAGGCACCATCAA3669830.24276987084223198No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGACTGTAGGCACCATCAAT3650190.24147062802625918No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGTAGGCACCATCAATAGA3470580.2295889069378237No Hit
TCCGGTCCTATTGTGTTGGCCTTCGGGATCGGCTGTAGGCACCATCAATA3431120.2269785137851614No Hit
CGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGGA3197480.21152255189494334No Hit
GGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAAT3158060.2089148048579959No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCCTGTAGGCACCATCAATAGA3157050.2088479904361969No Hit
TCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATAG2867020.1896616669170223No Hit
TAAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGACTGTAGGCACCATCA2865640.18957037592486126No Hit
AGGTAGCCGTACTGGAAGGTGCGGCTGGATCACCTGTAGGCACCATCAAT2843420.18810045864528308No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAAT2669910.17662227020335644No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA2482320.1642126490298159No Hit
AGCTAACGATGCGGGTTCGATTCCCGCTACCCGCCTGTAGGCACCATCAA2389850.15809549102609877No Hit
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCTGTAGGCACCATCAATA2364990.156450930109343No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGCTGTAGGCACCATCAATAG2326110.15387890563454554No Hit
TTGAGGCAATAACAGGTCTGTGATGCCCTTACTGTAGGCACCATCAATAG2295060.15182485831092254No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGACTGTAGGCACCATCAATA2252150.14898623767785776No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGACTGTAGGCACCATCAATA2226360.14728015457073257No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT2213560.1464333975419927No Hit
TTGAGGCAATAACAGGTCTGTGATGCCCTTCTGTAGGCACCATCAATAGA2175770.14393347972042383No Hit
CTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAATA2171110.14362520723964822No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCTGTAGGCACCATCAATAGA2125080.14058018958082807No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCTGTAGGCACCATCAAT2102180.1390652883340981No Hit
TGGGGATATAGCTCAGTTGGTAGAGCTCCGCCTGTAGGCACCATCAATAG2091010.13832636052073677No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACCTGTAGGCACCATCAATAG2080320.13761918609595322No Hit
TTGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAA2048080.1354864168298146No Hit
TCGTAGGTTCGAATCCTACTTGGGGAGCTGTAGGCACCATCAATAGATCG2027550.1341282979391872No Hit
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCACTGTAGGCACCATCAATAG1974890.13064468660063694No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATAG1966600.13009627911874214No Hit
TACAGACCCGGGTTCGTTTCCCGGCTGGTGCTGTAGGCACCATCAATAGA1955230.12934412072680676No Hit
AGTAAGCCCACCCCAAGATGAGTGCTCTCTCTGTAGGCACCATCAATAGA1912230.12649954633338362No Hit
ACGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT1911300.12643802414301425No Hit
TTCGGTGCGGGCTGCGCGAGCGGTACCAAATCGACTGTAGGCACCATCAA1910100.12635864067156988No Hit
TCGGTGCGGGCTGCGCGAGCGGTACCAAATCGACTGTAGGCACCATCAAT1893860.12528431769135612No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGAT1885340.12472069504410113No Hit
GCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAAT1867610.12354780425351063No Hit
TCGGGGCGTGGACTGTTGTCGGCCGCGCCGGCGGCTGTAGGCACCATCAA1862440.12320579379737115No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTTCTGTAGGCACCATCAATAGA1820690.12044391052003589No Hit
TCGCCCGCTGATCCGATGATTCATGATAACTCCTGTAGGCACCATCAATA1810280.11975525890525601No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCGCTGTAGGCACCATCAATAGA1788040.11828401856782042No Hit
ACCTTGAGGTCACGGGTTCGATTCCCGTCTCCGCCTGTAGGCACCATCAA1786300.1181689125342261No Hit
CCCGAGAGACCCGGGTTCGTGTCCCGGCAACGGACTGTAGGCACCATCAA1768010.11695897612362822No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGA1721030.11385111321658128No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA1714250.11340259660292061No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGCTGTAGGCACCATCAATAGAT1676560.1109092940706388No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGCTGTAGGCACCATCAATAGAT1665630.11018624295156638No Hit
CCGGGAGATGCCCAGCTTGAGAATCGGGCGGCCTGTAGGCACCATCAATA1632580.1079998898422028No Hit
ACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATAGA1628500.10772998603929196No Hit
CGTAGGTTCGAATCCTACTTGGGGAGCTGTAGGCACCATCAATAGATCGG1628350.1077200631053614No Hit
ACTCTGCTGGGCCGACACTGACACTGAGAGACGACTGTAGGCACCATCAA1626740.10761355694784021No Hit
ACGAGGCGCCGTCAACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGAT1594260.10546491098741269No Hit
TCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCCTGTAGGCACCATCAAT1576380.10428209726289164No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)