FastQCFastQC Report
Tue 22 Aug 2023
SRR5892474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5892474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144690174
Total Bases14.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT80056065.532929969384099No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT35956462.4850657792422033No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGGCTGTAGGCACCATCAATAGAT35809682.474921344693386No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAATA17668501.221126460183813No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAAT14208810.9820162356014582No Hit
TCGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGG11823880.8171861069155948No Hit
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA10633560.734919290372821No Hit
CGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGGA8363740.5780447813961438No Hit
CCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT8276080.5719863188498204No Hit
TCCGGTCCTATTGTGTTGGCCTTCGGGATCGGACTGTAGGCACCATCAAT6639710.45889156232544165No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG6497990.4490968405359717No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA6255110.43231062808729503No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATA6158670.4256453517016297No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAATA5915890.40886605057230774No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGAT5634170.3893954816862685No Hit
TACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCAATAG5589370.3862992106153663No Hit
TCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT5091220.3518704732499665No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAA4890730.3380139690757439No Hit
TCCTGCGGCAAAATAGCTCGATGCCAGAATCTGTAGGCACCATCAATAGA4554420.3147705109539781No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATA4479690.30960568200021654No Hit
TCGTAGGTTCGAATCCTACTTGGGGAGCTGTAGGCACCATCAATAGATCG4176520.2886526351125958No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAAT4014080.2774258879528336No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT3972870.2745777332467649No Hit
ACCGTCCACGCCCGGTCGTACTCATAACCGCACTGTAGGCACCATCAATA3957420.273509934406465No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCCTGTAGGCACCATCAATAGA3903590.26978957119783403No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA3860360.26680180784080054No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGTAGGCACCATCAATAGA3736330.2582296984451757No Hit
TCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATAG3687480.25485351894040853No Hit
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCTGTAGGCACCATCAATA3476340.24026095925491114No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTTCTGTAGGCACCATCAATAGA3456490.23888906236300467No Hit
CGTAGGTTCGAATCCTACTTGGGGAGCTGTAGGCACCATCAATAGATCGG3356140.23195355339057094No Hit
TCCGGTCCTATTGTGTTGGCCTTCGGGATCGGCTGTAGGCACCATCAATA2875330.1987232388012748No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA2794010.193102953902039No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGCTGTAGGCACCATCAATAGATC2778850.19205519788786765No Hit
CCGAGAGACCCGGGTTCGTGTCCCGGCAACGGACTGTAGGCACCATCAAT2759810.1907392826827342No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTTGCTGTAGGCACCATCAATAG2744710.18969567346017568No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGCTGTAGGCACCATCAATA2737480.18919598507083144No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGACTGTAGGCACCATCAAT2729310.18863133027955306No Hit
ACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATAGA2670410.18456056317963926No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGACTGTAGGCACCATCAATA2644540.18277260486258037No Hit
TCATCTCGGTTCGAGTCCGAGTGGCGGCACTGTAGGCACCATCAATAGAT2643120.18267446412774374No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAA2565830.17733270539850204No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGACTGTAGGCACCATCAATA2538230.17542518125660697No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGA2534910.17519572545403117No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGCTGTAGGCACCATCAATAG2529770.1748404836392No Hit
ACGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT2465560.17040272548155205No Hit
CCGAGAGACCCGGGTTCGGGTCCCGGCAACGGACTGTAGGCACCATCAAT2425890.1676610050935456No Hit
TACAGACCCGGGTTCGTTTCCCGGCTGGTGCTGTAGGCACCATCAATAGA2409580.16653376890679528No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT2381040.1645612783629661No Hit
TGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA2352410.1625825676317177No Hit
ACGAGGCGCCGTCAACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGAT2312760.15984222950758217No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGCTGTAGGCACCATCAATAGAT2206160.15247476307548016No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGCTGTAGGCACCATCAATAGAT2187320.15117267050905614No Hit
TAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGGAAG2131130.14728920016365452No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCGCTGTAGGCACCATCAATAGA2122120.14666649028979675No Hit
TCGCCCGCTGATCCGATGATTCATGATAACTCCTGTAGGCACCATCAATA2061010.14244298303214425No Hit
TCATCTCGGTTCGAGTCCGAGTGGCGGCCTGTAGGCACCATCAATAGATC1988200.13741085140999276No Hit
TGGGGATATAGCTCAGTTGGTAGAGCTCCGCCTGTAGGCACCATCAATAG1835950.12688836769247372No Hit
GTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGGAA1827530.1263064346028086No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAGA1819330.12573970641572385No Hit
CCGGGAGATGCCCAGCTTGAGAATCGGGCGGCCTGTAGGCACCATCAATA1810590.12513565710412375No Hit
TCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCCTGTAGGCACCATCAAT1780240.12303807167997462No Hit
TCGACCCCAGGTCAGGCGGGACTACCCGCTGACTGTAGGCACCATCAATA1779250.12296964961836317No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACCTGTAGGCACCATCAATAG1766580.12209398545612365No Hit
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCACTGTAGGCACCATCAATAG1732510.11973929895197996No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATAG1693650.11705356025074655No Hit
ACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAATAGA1689400.11675982917817211No Hit
GCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAAT1619080.11189978940795246No Hit
ACGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGG1617520.11179197282601928No Hit
GCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT1613110.11148718364247734No Hit
AGGTAGCCGTACTGGAAGGTGCGGCTGGATCACCTGTAGGCACCATCAAT1602380.11074559907571886No Hit
CTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAATA1593480.11013049165315124No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAAT1591940.11002405733508897No Hit
ACGGAGGGACGGAGGAGGCTAGGTTAGCCGACTGTAGGCACCATCAATAG1582630.10938061350316713No Hit
AGTAAGCCCACCCCAAGATGAGTGCTCTCTCTGTAGGCACCATCAATAGA1575990.10892170189801555No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCTGTAGGCACCATCAATAGA1575260.1088712492667263No Hit
ACTCTACTGCGGTGACGATACTGTAGGGCTGTAGGCACCATCAATAGATC1534710.10606870926839855No Hit
TTGAGGCAATAACAGGTCTGTGATGCCCTTCTGTAGGCACCATCAATAGA1529010.10567476406518109No Hit
TCTACGGTTCGAATCCGTATAGCCCCTGTAGGCACCATCAATAGATCGGA1525140.1054072960061545No Hit
TTGAGGCAATAACAGGTCTGTGATGCCCTTACTGTAGGCACCATCAATAG1497570.10350184526006583No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCTGTAGGCACCATCAATAGAT1492990.10318530683362093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)