FastQCFastQC Report
Fri 14 Jul 2023
SRR5892475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5892475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142674937
Total Bases14.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT2258544815.830003835922493No Hit
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA50418093.5337734195039454No Hit
CCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT28297601.9833616607799869No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA21645891.517147331910159No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA20297611.422647202570694No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGGCTGTAGGCACCATCAATAGAT16674351.1686951016491425No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAAT16449901.1529635369665523No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAATA16082411.1272063852391958No Hit
TCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATAG11864150.8315510943593408No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT11631520.8152461984265674No Hit
GCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT10706960.7504443474890057No Hit
GCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAAT10692710.749445573611853No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATA10230000.7170145097032704No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATA7850210.5502164686429825No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA7814150.5476890450633246No Hit
TTGCCGGCCGGGGGACGGACCGGGAACGGCCCCCCTGTAGGCACCATCAA7293560.51120120697863No Hit
ACGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT7236130.5071759730302177No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAATA7137340.5002518417092414No Hit
CCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATAGAT5751480.40311775290988916No Hit
CGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGGA4626050.324237045221194No Hit
ACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATAGA4555590.31929854645739214No Hit
TGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA4424620.3101189384089232No Hit
TCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAAT4351450.3049904973849752No Hit
TTCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATA3885020.27229870092740954No Hit
ACCGTCCACGCCCGGTCGTACTCATAACCGCACTGTAGGCACCATCAATA3866720.27101606500096087No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT3623880.253995556346382No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGAT3597920.25217603565509195No Hit
GCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATAGA3284250.23019109516060274No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAAT3197790.22413116607859443No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATAG3189920.22357956254082664No Hit
TCGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGG3180500.2229193204409826No Hit
TTGCCGGCCGGGGGACGGACCGGGAACGGCCCCCTGTAGGCACCATCAAT3041360.21316708203627943No Hit
CTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAATA3036390.21281873774368654No Hit
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCTGTAGGCACCATCAATA2825000.19800254055833225No Hit
TCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT2691240.18862738309812604No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG2600320.1822548553149177No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGCTGTAGGCACCATCAATAGATC2591360.18162685433672207No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT2549820.1787153408730785No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCTGTAGGCACCATCAATAGAT2416040.16933878162497454No Hit
GTCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATA2282530.1599811465134903No Hit
AGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATAGATCGG2247330.15751399981343606No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGACTGTAGGCACCATCAAT2164780.15172812026543947No Hit
ACCTCCTGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATAG2017310.1413920372013201No Hit
TTTACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAAT1898510.13306541708863695No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCCTGTAGGCACCATCAATAGA1897650.13300514020903334No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGACTGTAGGCACCATCAATA1896630.13293364902624769No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGTAGGCACCATCAATAGA1862420.13053589082713243No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGCTGTAGGCACCATCAATAGAT1771890.12419069790793039No Hit
TCGCCCGCTGATCCGATGATTCATGATAACTCCTGTAGGCACCATCAATA1745410.12233473073129865No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGCTGTAGGCACCATCAATAG1716040.12027620520344089No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGCTGTAGGCACCATCAATAGAT1706670.11961946757334121No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAAT1698570.11905174347474917No Hit
TACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCAATAG1675900.11746281689264038No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCTGTAGGCACCATCAATAGATC1585870.11115266867088226No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCCTGTAGGCACCATCAATAGATC1563720.10960018857411516No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCCTGTAGGCACCATCAATAGATC1528360.10712182757087883No Hit
TAGTCTTTGAACGCAAGTTGCGCCCGAGGCCCTGTAGGCACCATCAATAG1493600.10468552020457525No Hit
CCGAGAGACCCGGGTTCGTGTCCCGGCAACGGACTGTAGGCACCATCAAT1474750.10336433511093823No Hit
TTTAGTTGGTGGAGCGATTTGTCTGGTTCTGTAGGCACCATCAATAGATC1452530.10180694875652897No Hit
TTGGCTGTAGTTTAGTGGTGAGAATTCCACGCTGTAGGCACCATCAATAG1439140.10086845175897993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)