FastQCFastQC Report
Fri 7 Jul 2023
SRR5892476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5892476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140143588
Total Bases14 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT139448319.950388169025613No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAATA35048112.5008714633451516No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAAT26239621.8723382478262225No Hit
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA24385361.7400268073627458No Hit
CCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT19772901.4109029376356486No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA18684321.3332268901235782No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT18502791.3202737466661694No Hit
TCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAATA18401621.3130547221325601No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGGCTGTAGGCACCATCAATAGAT15214911.085665795855034No Hit
TCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATAG14353471.0241974110153367No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATA12353210.8814680840053846No Hit
GCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAAT9047640.6455978563928304No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA9026390.6440815544126072No Hit
TGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATA8841390.6308808077612513No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAATA8356920.5963112632737789No Hit
TTGCCGGCCGGGGGACGGACCGGGAACGGCCCCCCTGTAGGCACCATCAA8082730.5767463296287234No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAA6011070.4289222279652209No Hit
GCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT5646260.4028910691226201No Hit
TTCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATA5348560.38164857032203287No Hit
ACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAATAGA5173610.36916494531308847No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAAT5075300.3621499971871706No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT4798800.3424202325974414No Hit
ACGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAAT4301580.30694090692183507No Hit
TCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCAAT4235210.302205049866427No Hit
ACCGTCCACGCCCGGTCGTACTCATAACCGCACTGTAGGCACCATCAATA4214580.30073298822633254No Hit
TCGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGG4194820.2993230057731931No Hit
CGTAGGTTCGAATCCTACTTGGGGACTGTAGGCACCATCAATAGATCGGA4118150.2938521882285474No Hit
TCGACCCCAGGTCAGGCGGGACTACCCGCTGAGCTGTAGGCACCATCAAT4091110.2919227385558303No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAAT3999810.28540799169491793No Hit
CTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAATA3925590.2801119948491686No Hit
GTCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAATA3642630.25992127445745145No Hit
TTACGGAGGGACGGAGGAGGCTAGGTTAGCCGACTGTAGGCACCATCAAT3595340.25654687819181565No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGAT3478160.24818545390745952No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG3413100.24354307240942052No Hit
TCGGCCGAGGGCACGCCTGCCTGGGCGTCACGCTGTAGGCACCATCAATA2974140.21222091159818174No Hit
CCGAGAGACCCGGGTTCGTGTCCCGGCAACGGACTGTAGGCACCATCAAT2921820.20848759773440367No Hit
TGGCCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATA2906200.20737302658470538No Hit
TCCGGTCCTATTGTGTTGGCCTTCGGGATCGGACTGTAGGCACCATCAAT2821200.20130781866381214No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGTAGGCACCATCAATAGA2739810.19550020369108861No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGCTGTAGGCACCATCAATAGAT2717810.1939303851703868No Hit
GCGCATTTATTAGATAAAAGGCTGACGCGGGCTGTAGGCACCATCAATAG2704600.1929877805040927No Hit
TTGCCGGCCGGGGGACGGACCGGGAACGGCCCCCTGTAGGCACCATCAAT2676070.1909520113042917No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGCTGTAGGCACCATCAATAGAT2589460.184771921209838No Hit
TTTACGGAGGGACGGAGGAGGCTAGGTTAGCCGCTGTAGGCACCATCAAT2571960.18352320193200705No Hit
CCGAGAGACCCGGGTTCGGGTCCCGGCAACGGACTGTAGGCACCATCAAT2435220.1737660662719724No Hit
TGTCCCGGCCCCGAACCCGTCGGCTGCCGGCGGCTGTAGGCACCATCAAT2247720.16038693115235497No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGACTGTAGGCACCATCAATA2239610.15980823896131446No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGCTGTAGGCACCATCAATAGATC2201980.15712313573704137No Hit
TGGCGCATTTATTAGATAAAAGGCTGACGCTGTAGGCACCATCAATAGAT2165160.154495830376485No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGCTGTAGGCACCATCAATAG2145450.1530894156927108No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCCTGTAGGCACCATCAATAGATC2090660.14917985402229034No Hit
TCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCCTGTAGGCACCATCAAT2041810.14569414335245934No Hit
TTCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCCTGTAGGCACCATCAA2014770.14376469367974223No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCCTGTAGGCACCATCAATAGATC2008840.1433415562330258No Hit
TTGGCTGTAGTTTAGTGGTGAGAATTCCACGCTGTAGGCACCATCAATAG1920120.13701090627135934No Hit
CCGAGAGACCCGGGTTCGTGTCCCGGCAACGGCTGTAGGCACCATCAATA1918140.13686962260449617No Hit
TCTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAAT1904430.13589134024454974No Hit
ACGGAGGGACGGAGGAGGCTAGGTTAGCCGACTGTAGGCACCATCAATAG1862200.13287800223867538No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT1836230.1310249028303742No Hit
ATTTATTAGATAAAAGGCTGACGCGGGCCTGTAGGCACCATCAATAGATC1820880.12992959763524822No Hit
CCGTTGTAGTCTAGCTGGTTAGGATACTCGGCTGTAGGCACCATCAATAG1819910.12986038290956273No Hit
CCGAGGGCACGCCTGCCTGGGCGTCACGCCTGTAGGCACCATCAATAGAT1795490.12811788435158375No Hit
TGGCCGCCGCGTGCCGGCCGGGGGACGGACCGGGACTGTAGGCACCATCA1738160.12402707999740951No Hit
TAAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGACTGTAGGCACCATCA1704260.12160813236778269No Hit
TAGTCTTTGAACGCAAGTTGCGCCCGAGGCCCTGTAGGCACCATCAATAG1693530.12084248906200404No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCCTGTAGGCACCATCAATAGA1690120.12059916719129526No Hit
TCCCGGCCCCGAACCCGTCGGCTGCCGGCGGACTGTAGGCACCATCAATA1669120.11910070405789809No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAGA1642620.1172097862943255No Hit
ACTCTGCTGGGCCGACACTGACACTGAGAGACGCTGTAGGCACCATCAAT1625430.11598318718655898No Hit
AGGTAGCCGTACTGGAAGGTGCGGCTGGATCACCTGTAGGCACCATCAAT1608730.11479155221857172No Hit
CCGAGAGACCCGGGTTCGGGTCCCGGCAACGGCTGTAGGCACCATCAATA1585590.11314038855634266No Hit
TCCCGGCCCCGAACCCGTCGGCTGCCGGCGCTGTAGGCACCATCAATAGA1585010.11309900243170598No Hit
CCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCCTGTAGGCACCATCAA1522940.10866997354170782No Hit
AGTAAGCCCACCCCAAGATGAGTGCTCTCTCTGTAGGCACCATCAATAGA1477200.1054061781263942No Hit
TCGAGGCGCCGTCAACGAGTCGGGTTGTTTCTGTAGGCACCATCAATAGA1475080.10525490470530839No Hit
TCCGGTCCTATTGTGTTGGCCTTCGGGATCGGCTGTAGGCACCATCAATA1458370.10406255618344808No Hit
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCACTGTAGGCACCATCAATAG1439720.1027317782102168No Hit
TCGCCCGCTGATCCGATGATTCATGATAACTCCTGTAGGCACCATCAATA1414140.10090650740296446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)