FastQCFastQC Report
Mon 25 Sept 2023
SRR592954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23192119
Total Bases927.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA278883912.02494261089295Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA18456847.958237882446189Illumina Single End Adapter 1 (95% over 22bp)
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA5168892.2287269222790727Illumina Single End Adapter 1 (95% over 22bp)
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA4744502.045738037132355Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG4262711.8379993652153992Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA3101581.337342223882173Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2650771.142961537925879No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1654430.7133587060328554No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG1302380.5615614511119057No Hit
AGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA1297110.559289127483349Illumina Single End Adapter 1 (95% over 22bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT1093330.47142307263946No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC1022170.4407402359396311No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA920050.3967080368982239No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG879230.3791072303483783No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA727470.31367120874121074No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG637540.27489510553132296No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA630630.27191564513790223No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG508930.21944092301354612No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG484960.20910551554172346Illumina Single End Adapter 1 (95% over 22bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG484130.20874763534974966No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG482760.20815691744251572No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA465740.20081821760228122No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG459760.19823975549625286No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGC439650.18956870650758562No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA426660.18396766591271804No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC422670.18224725390551852No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAG419910.18105719447196697No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG378500.16320199115915196No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA358700.15466460826628217Illumina Single End Adapter 1 (95% over 22bp)
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC353600.15246558539993696No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA352040.15179294311140779Illumina Single End Adapter 1 (95% over 22bp)
AACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT327540.14122900973386693Illumina Single End Adapter 1 (95% over 21bp)
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA322530.13906879315339837Illumina Single End Adapter 1 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG307290.13249759541161374No Hit
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTT302000.1302166481639733No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT300210.12944483425598152No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC284440.12264511060847867No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAG250040.10781248578450292No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC245700.10594116044333854No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG241910.10430698462697609No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT241150.10397928710179523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)