FastQCFastQC Report
Mon 25 Sept 2023
SRR592955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26395689
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA2107150.7982932364447847No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG1924450.7290773883568639No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1859610.7045127710059018No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1383440.5241158887725946No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC1067940.4045887947838755No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGC994870.3769062440461395No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC923550.34988668035905407No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG891070.3375816406989793No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA888680.3366761898126622Illumina Single End Adapter 1 (95% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG779460.2952982208572013No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA767010.29058154155400145No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG689460.2612017439666No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC631060.23907691896203204No Hit
ATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCA596220.22587779390793702No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATT528210.20011222287093927No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA522310.1978770093858887No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAG488820.18518933148515276No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCT479840.1817872607909572No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC479140.18152206597069695No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC466080.17657428832412747No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTA465510.176358343970487No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA442500.16764101137878992No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGG403080.15270675450070653No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA399850.15148306983007717Illumina Single End Adapter 1 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC385610.1460882494864976No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCA364500.13809073140693542No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCTGTAG361220.13684810424914462No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG360300.13649956248537404No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC344170.13038871612709182No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCA318150.12053104580827573No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC316170.1197809233166825No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCA309770.11735628496001753No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTA300530.11385571333258244No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG292610.11085522336620954No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC287180.1087980692604766No Hit
ATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTC285630.10821085215847179No Hit
CAAGACCTCAGCATGGCTCGGAGGCTATGGATCTGTAGGC283500.10740390220539421No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCT276380.10470649203360442No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG271940.1030243991736681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)