FastQCFastQC Report
Mon 25 Sept 2023
SRR592956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31785837
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT17356185.460350155322321No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT12194493.8364539527463126No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT4563421.435677153947527No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4407861.3867371181699573No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4393731.3822917420736789No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG2689670.8461850477619954No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2518620.7923717723714496No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2286280.7192763242320785No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC1954320.6148398734946008No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCG1883380.5925217574103837No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1698020.5342064769287025No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1592610.5010439083293606No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1553970.4888875507667141No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1328150.4178433306632762No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1322860.41617906742553296No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1279950.40267934426266644No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG1273350.4006029477845746No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1215280.3823338048326366No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1203980.37877876237772184No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT1133300.35654244373052063No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT875630.2754780375926549No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT862610.2713818736313283No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG855780.2692331178820303No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA848850.2670529015800339No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT823700.2591405725763962No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA818200.25741024217798636No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA808960.2545032871086579No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA804370.2530592477398031No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT731570.23015596537539657No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT692020.21771331678319497No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC652370.205239207638295No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA624880.19659070170151569No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC623050.19601497358713568No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC620130.19509632544834354No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT616430.1939322849985042No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA595040.18720287277632489No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA562480.17695931681773866No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATTCGTA521510.16406992837722031No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT514770.16194948712535084No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT511780.16100881659966987No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAAT484160.15231941194438264No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA474780.14936841210127644No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA453830.1427774263109699Illumina Single End Adapter 1 (95% over 22bp)
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTCG451230.14195945194081253No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC441220.13881025061570662No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCA437600.13767137860802595No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC419480.13197072645908303No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT419190.13187949085625778No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTCGT408600.12854781832550138No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA399930.1258201884065535No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT390650.12290064911614565No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATT390500.12285345828709812No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATTCG388110.12210155107760728No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCG383060.1205127931663401No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA376310.11838920585920075No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA367890.11574022732199879No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC367560.1156364074980942No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA347660.10937575751112044No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA340060.10698475550604504No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT336650.10591195065903095No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC328890.10347061176963815No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATTCG322000.10130297968872111No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT319000.1003591631077703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)