FastQCFastQC Report
Mon 25 Sept 2023
SRR592957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25038467
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC12208984.876089258978994No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC8731573.4872622193683025No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG8590033.4307331994406844No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC6737612.6909035605095153No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG5172012.065625663104694No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC3371751.3466279704743904No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC2533521.0118510849725744No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG2477660.9895414124195383No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA2405980.9609134616747903No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAG2128170.8499601832652135No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1873460.7482327092948622No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG1852440.7398376266406406No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG1630550.6512179839125135No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCA1437040.5739329009240063No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG1412470.5641199998386482No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1403780.5606493400734158No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCA1398590.5585765294656417No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACC1342810.5362988077504904No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG1327550.5302041854239719No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT1302600.5202395178586612No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG1250030.499243823513636No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGC1237340.49417562185416547No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG1196470.4778527375497869No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC1129930.4512776281391349No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC977190.3902754909076502No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC886330.3539873267800301No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGG873590.3488991558468815No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCA854640.3413308011229282No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA813300.32482020564597663No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACC797080.3183421732648409No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG761380.30408411185876516No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT732700.29262973647707746No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA698510.2789747471360767No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG684580.2734113074893922No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG659570.26342267679566805No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA641670.25627367681895225No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAG625650.24987552153252832No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAG622090.24845370924665636No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGG617140.2464767511525366No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGC601630.24028228245762812No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC594150.2372948791154027No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCAC588800.23515816683185917No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC587800.2347587813582996No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC579100.2312841277383316No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC578010.23084879757215168No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATC574570.22947491154310684No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC554890.22161500542345505No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGG552810.22078428363845118No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCA535380.21382299483430836No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC515310.20580732837996832No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA488020.19490809880652837No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA482950.1928832144555815No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCA465970.18610164911454044No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC446860.17846939271481757No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT443740.1772233100373118No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGC442380.17668014579327082No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC442050.17654834858699617No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC435750.17403222010357103No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTG435240.17382853351205568No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC427880.17088905642665742No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA425530.16995050056379252No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGG424180.16941133017448712No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG404450.16153145478115732No Hit
TTAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGG395450.15793698551912144No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAG379500.15156678721584674No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCA379290.1514829162663992No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGC378310.15109151850231087No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG375710.15005311627105605No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTA371850.1485114883431162No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC371070.14819996767373977No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGG370220.14786049002121415No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC364650.1456359129334875No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGT364320.14550411572721286No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTA361910.14454159673593434No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGG352130.14063560680452203No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC342670.13685742022464872No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTA338280.13510411799572236No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC335720.13408169118340993No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGC335150.13385404146348098No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGC334080.13342669900677226No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT319700.12768353589698603No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAG316630.12645742249315822No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGC316170.12627370517532083No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCAC312590.12484390517997768No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGG312280.1247200956831742No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGC302260.12071825323810759No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGC291550.11644083481628488No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAG290380.1159735538122202No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCTGTAG281390.11238307840491991No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTA280360.11197171136715359No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGC270770.10814160467571755No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGCTGTAGGCAC265400.10599690468270281No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGG259380.10359260413187438No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGG256400.10240243542066693No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC254720.1017314678250869No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGC254170.10151180581462915No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGC253360.10118830358104591No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGC251600.10048538514758112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)