FastQCFastQC Report
Mon 25 Sept 2023
SRR592958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26145020
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC11362034.345772158521967No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC9011673.4468017236169644No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG7669202.93333108943883No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC5150641.970027179172171No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG4078481.55994525917364No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG2632101.0067309185458644No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC2525350.9659009631662167No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGG2218240.8484369107386416No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG2131410.815225997149744No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA2109820.8069682103895884No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG1789460.6844362712287081No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG1706970.652885329596229No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1650480.6312789204215564No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC1613170.617008516344604No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC1501370.5742470267760361No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAG1392520.5326138591594116No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG1373650.5253964234871498No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG1188590.45461430130862396No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA1144050.4375785522443662No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG967730.3701393228997339No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG950500.363549157736349No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACC838890.3208603397511266No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT814950.3117037202495925No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC803920.30748494359537687No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGG775540.29663010393566347No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC733900.2807035527224687No Hit
TATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAG722480.2763356080813861No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAG709090.2712141738656157No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT590330.22579060945449647No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGC573460.2193381378174505No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATC539010.2061616323108569No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG536760.20530104777123906No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC536590.20523602582824566No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG522620.19989275204226276No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC520950.19925400707285745No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGC517150.1978005754059473No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC504190.19284360845774837No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC495450.18950071562385495No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCA484200.1851977929257656No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA476990.18244009757881233No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT450310.1722354773490324No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCA448410.17150876151557734No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAG446750.17087384136634814No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGC443260.16953897912489643No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC443240.1695313294845443No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA432670.16548849455842835No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA421100.16106317761470443No Hit
TGATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTA415620.1589671761582129No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA391940.14991000198125684No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA390800.1494739724811838No Hit
CAAGACCTCAGCATGGCTCGGAGGCTATGGACTGTAGGCA385930.1476112850554331No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCAC381620.1459627875595429No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGG375350.1435646253091411No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTA370390.1416675145018057No Hit
CAAGACCTCAGCATGGCTCGGAGGCTATGGCTGTAGGCAC370300.141633091120221No Hit
ATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCA359990.1376897015186831No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC355380.1359264594175105No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA353860.1353450867507464No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGC353760.13530683854898562No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA346450.13251089500027155No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG342160.1308700471447335No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG337590.1291221043242652No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCA331320.1267239420738634No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTA329680.12609667156498636No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCA327740.12535465645082697No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCA319340.12214180750292024No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC316690.12112823015625922No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT314170.12016437547188719No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGG313140.11977041899375102No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCA309210.1182672646645518No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCAC286440.10955814912361896No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC285810.10931718545252596No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC284780.10892322897438976No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCTGTAG281520.10767633759698789No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAG276200.10564153326331363No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGC273780.10471592678070242No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAG273510.10461265663594826No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC272430.1041995760569317No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACC266870.10207297603903154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)