FastQCFastQC Report
Mon 25 Sept 2023
SRR592959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55983543
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT10567051.8875279115507213No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT10192181.820567162031885No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6583731.1760116718586389Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT5737481.0248511781399758No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT5015120.8958204020778034No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT4683420.836570847257738No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG3866900.6907208427305146No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATTCG3542210.6327234416014006No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT3402000.607678581543151No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATTCG3353710.5990528323653971No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC3014190.538406438477822No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2794180.4991073894697947No Hit
TCGCGCCGGGCCGTACCCATATCCGCAGCCTGTAGGCACCATCAATTCGT2644450.47236202967718566No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2533440.45253298813188725No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT2506080.44764583763482063No Hit
TAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATTCGTAT2474470.44199953547062926No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2352900.42028422531242793No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATTC2195130.3921027291895406No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT2155120.38495598608326737No Hit
TCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATTCGTA2137670.38183899865001397No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT2040200.3644285250042142No Hit
CTCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATTCGT2036130.3637015256429912No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA1982390.3541022760921009No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGCTGTAGGCACCATCAATTCGTAT1943010.34706806605648377No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC1937140.34601954363624327No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT1819860.32507053010203374No Hit
TTAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATTCGTA1714190.30619534029848733No Hit
AATTATTCCCCATGAACGAGGAATTCCCCTGTAGGCACCATCAATTCGTA1691500.3021423635156496No Hit
TAAGCCATGCATGTCTAAGTACGCACGGCCTGTAGGCACCATCAATTCGT1642210.2933379904162193No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1550380.27693495568867443No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACTGTAGGCACCATCAATTCGTA1500610.26804484310683946No Hit
TAGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATTCGTATG1499560.2678572879890792No Hit
TGTACAGTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATTCGTA1499070.2677697622674578No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCG1493000.26668551506288196No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACCTGTAGGCACCATCAATTC1435530.2564199982841386No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT1432750.25592342378187816No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATTCGTAT1417620.2532208438469141No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCCTGTAGGCACCATCAATTCG1403740.2507415438140455No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATTCGT1401290.25030391520593825No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTC1397640.24965193789181936No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1313690.23465645966708465No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATCTGTAGGCACCATCAATT1293030.23096608944525002No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1261370.22531085608497484Illumina Single End Adapter 1 (95% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1238060.2211471324706977No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATTCG1235480.22068628275277255No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATTCGTA1223020.21846062868868446No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATTCGTATG1220000.21792118444522168No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1173060.20953657756173097No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATTC1135290.20278995204001288No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATTCG1124420.2008483100113903No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG1124220.20081258522705506No Hit
TAGACCGTCGTGAGACAGGTTAGTTTTACCCTCTGTAGGCACCATCAATT1113640.19892274413571862No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG1081880.1932496483832758No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACCTGTAGGCACCATCA1067780.19073105108763838No Hit
TGGCCATGATTAAGAGGGACGGCCGGGGGCCTGTAGGCACCATCAATTCG1054210.18830712447048947No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1048600.1873050442698848No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1040740.18590106024550823No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCCTGTAGGCACCATCAATTCG1023770.18286981229465954No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATT1013540.18104248957590982No Hit
TTCTCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATTC1005860.179670657857435No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT988910.17664298238501983No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT987200.17633753547895317Illumina Single End Adapter 1 (95% over 22bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC982360.17547299569803934No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATTCG958480.17120745644840663No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATTCG943500.16853167010169398No Hit
TAGACGTGGCGACCCGCTGAATTTAAGCCTGTAGGCACCATCAATTCGTA921160.16454121169144298No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATTC915080.16345517824765038No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA914920.16342659842018215Illumina Single End Adapter 1 (95% over 22bp)
AATTATTCCCCATGAACGAGGAATTCCCAGTCTGTAGGCACCATCAATTC913430.16316044877688432No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG907700.16213693370567847No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACCTGTAGGCACCATCAATTCGTA892710.15945936111974907No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT884080.15791783667568166No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG869090.15524026408975225No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATTCGTA845630.15104974688722364No Hit
TAGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATTC844600.1508657642478969No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT815380.1456463732565122No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT810120.14470681142849426No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT806540.14406733778889272No Hit
CCCCTGTAATTGGAATGAGTCCACTTTCTGTAGGCACCATCAATTCGTAT790850.1412647284577898No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCCTGTAGGCACCATCAATTCGT760100.13577204286624017No Hit
CCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT751710.13427338816337508No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATTCGT746390.13332310890005658No Hit
TATGCTTGTCTCAAAGATTAAGCCATGCCTGTAGGCACCATCAATTCGTA715800.12785900313597517No Hit
AGGGGCGAAAGACTAATCGAACCATCTCTGTAGGCACCATCAATTCGTAT707540.126383569542928No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATTCGTA706480.126194228185951No Hit
CCCTGTAATTGGAATGAGTCCACTTTCTGTAGGCACCATCAATTCGTATG693050.1237953089178368No Hit
AGGGGAATCCGACTGTTTAATTAAAACCTGTAGGCACCATCAATTCGTAT692020.1236113262785101No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGGGCTGTAGGCACCATCAATTCGT689920.12323621604298962No Hit
GGTACACCTGTCAAACGGTAACGCAGGTGTCCTCTGTAGGCACCATCAAT684940.1223466689130411No Hit
AGTGAAACTGCGAATGGCTCATTAAATCCTGTAGGCACCATCAATTCGTA662240.11829190589098657No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCGT649870.11608232797984937No Hit
ACCCGGCCCGGACACGGACAGGATTGACCTGTAGGCACCATCAATTCGTA643120.11487661650853359No Hit
TGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATTCG639930.11430680619838583No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCTGTAGGCACCATCAATTCGTA636690.11372806469215427No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCCTGTAGGCACCATCAATTC633040.11307608737803536No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG627280.11204721358917924No Hit
TCATGATTAAGAGGGACGGCCGGGGGCCTGTAGGCACCATCAATTCGTAT625810.11178463642431491No Hit
TAATCGAACCATCTAGTAGCTGGTTCCCTCCCTGTAGGCACCATCAATTC624430.11153813541240146No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC600160.10720293283331497No Hit
CGCGCCGGGCCGTACCCATATCCGCAGCCTGTAGGCACCATCAATTCGTA599410.10706896489205764No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTCTGTAGGCACCATCAATTC597370.10670457209183777No Hit
TATAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCA591930.1057328579579181No Hit
TTCGCGCCGGGCCGTACCCATATCCGCAGCCTGTAGGCACCATCAATTCG585570.10459680981605612No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT578390.10331429005841948No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATTCGTATG576590.10299276699940194No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCCTGTAGGCACCATCAATTCGT563300.10061885508032245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)