FastQCFastQC Report
Mon 25 Sept 2023
SRR592960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46160824
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT18577674.024553374523817No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT15981173.462063415505755No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT13613522.949150127822675No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9256902.00535848320212No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG7717721.6719198946708578No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4654641.0083528838220046Illumina Single End Adapter 1 (95% over 22bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4243490.9192838498723507No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT3859650.8361310881278896No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT3778640.8185815747136576No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3759660.8144698630163101No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC3756140.8137073116372446No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3485270.7550276832146671No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC3393430.7351320245063216No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3243300.7026087749213489No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3010210.6521135757888551No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2613360.5661424068166547No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT2567010.556101424879244No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG2229190.4829181558804063No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT2192690.47501101800089185No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT2152070.4662113483936075No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG1983760.42974969424289305No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG1939910.4202502970917504No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1842770.3992064786365166No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT1712960.37108523019433104No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1552720.33637181173369No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1523760.3300980935695602No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1330000.28812310629463633No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1305170.2827440861974214No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC1214420.2630845584558889No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC1174190.25436937607526244No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1145020.24805016478908606No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1105890.23957327971441755No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATTCGTA1090990.2363454343882596No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1072570.23235503768303617No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA1062580.23019086487710877No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC1057050.22899287932988374No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA991830.2148640154257212No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT986770.2137678478183145No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT938670.203347756530516No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT912290.19763295386581486No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA851990.184569928821028No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT789420.17101514479030963No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCTGTAGGCACCATCAATT743230.16100882427921998No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT705280.15278756722367No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTA704060.15252327384797118No Hit
CAAGACCTCAGCATGGCTCGGAGGCTATGGCTGTAGGCACCATCAATTCG673300.1458596146377283No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATT659390.14284623688693251No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATTCGTA640540.13876268759847096No Hit
CAAGACCTCAGCATGGCTCGGAGGCTATGGACTGTAGGCACCATCAATTC624960.13538753121044805No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT610560.13226800284154372No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA598820.12972472068522867Illumina Single End Adapter 1 (95% over 22bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA593630.12860039066893608No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATTCGTA592030.1282537764057245No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT584140.1265445348202623No Hit
CAAGACCTCAGCATGGCTCGGAGGCTATGGATCTGTAGGCACCATCAATT577840.12517974115886665No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATTCGT565190.12243932214035No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG564270.12224001893900334No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT560060.12132799015892784Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT552790.11975306160046018No Hit
TCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAAT524030.11352267021923178No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATTC520700.11280127928392267No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT512450.11101404948923789No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT509650.11040747452861759No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA494280.10707781126264124Illumina Single End Adapter 1 (95% over 22bp)
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA493370.10688067440043966No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT493000.10680051985207198No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC482730.10457568955008255No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATTCGTAT477080.10335170793311663No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT476080.10313507401860937No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTA465340.10080842577680155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)