FastQCFastQC Report
Mon 25 Sept 2023
SRR592961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21624068
Total Bases864.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC1258060.5817869237185158No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA663350.30676466611185277No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAG519930.2404404203686374No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA408000.1886786519539247No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGC401190.18552938327792903No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA379960.1757116191088559No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG348530.1611768886409347No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCAC345190.15963231340190015No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAG321410.14863530765811503No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA309640.14319229850738538No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC264640.12238215307129076No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC248740.11502923501720398No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCA235020.1086844528975769No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG234930.10864283260670472No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC234830.10859658783906895No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAG230440.10656644253985884No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA227290.1051097323593322Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC222270.10278824502401676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)