FastQCFastQC Report
Mon 25 Sept 2023
SRR592962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR592962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20673613
Total Bases826.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC2500581.2095515186435966No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1445160.6990360127182415Illumina Single End Adapter 1 (95% over 22bp)
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA1257150.608093998857384No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC1115920.5397798633456088No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC998330.4829005941051523No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC848350.41035401020615025No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCAC759010.3671395029015973No Hit
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT651350.3150634579451594Illumina DpnII expression PCR Primer 2 (95% over 24bp)
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC649120.31398478824190046No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGG630780.30511357642227316No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCA519620.25134455211094453No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCAC514270.2487567122398973No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA481800.23305070091038274Illumina Single End Adapter 1 (95% over 22bp)
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA468320.22653031185211797No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCA464020.22445036578753796No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA453830.21952137732287047No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG428200.20712393136119944Illumina Single End Adapter 1 (95% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCAC411650.19911855755450195No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACC402920.19489578333501745No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCA401610.19426212534790122No Hit
TCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCA397530.19228859512848576No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGG390940.18910095685742012No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC390030.18866078222514854No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA385420.18643088656056392No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC354120.17129081404396995No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCAC347230.16795806325677085No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCA345850.1672905456825568No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC341420.16514771752765228No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACC338820.16389007572116204No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA312920.15136202849497085No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCAC308450.14919985200458188No Hit
CTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTATG303000.1465636412948235Illumina DpnII expression PCR Primer 2 (95% over 23bp)
TGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTATGC293860.1421425466366232Illumina DpnII expression PCR Primer 2 (95% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGC284470.13760052488164504No Hit
TGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCA282540.13666696769451958No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCA279370.13513361210737573No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA272690.13190244008147003Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAG271270.13121557417177152No Hit
TGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCAC267400.12934362271364952No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACC258210.12489834263609365No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAG251500.12165265935857462No Hit
TTGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA251180.1214978726746989No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAG248220.12006609584884849No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACC247080.11951466828754123No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACC242770.1174298851390901No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGC236730.11450828648093586No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACC231770.1121090928808622No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC225180.10892145460979655No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCAC221210.1070011323129634No Hit
TCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACC214700.10385219071286668No Hit
CCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA211880.10248813306121189No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACC210200.10167550297086436No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCA210120.10163680629989542No Hit
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTT209130.1011579349966549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)