FastQCFastQC Report
Thu 6 Jul 2023
SRR5933683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51309324
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA14593312.844182862358506No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA8608031.6776736329638644No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA7904121.5404841428041423No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA7587681.478811141616288No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA6985191.3613880393356965No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA5932201.1561641311041244No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA4881230.9513339135007898No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA4735340.9229004849099162No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA3846040.7495791603101222No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAAA3804710.7415240941393031No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA3738620.7286433943273157No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA3265110.6363580233487387No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA2976000.5800115394231271No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA2899800.5651604375064462No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA2645950.5156859989034351No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA2553260.4976210561651524No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAA2218320.4323424724909648No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA2203580.42946970028293496No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAAA2147200.418481444035396No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAT2092110.4077446040801473No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1924570.37509166949851064No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA1824080.35550653522544945No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA1692450.32985232859431163Illumina PCR Primer Index 2 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1618300.31540076419638663No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAAAAAA1575420.3070436086821179No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA1571490.3062776660242103No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA1299150.25319959389837216No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA1267860.24710128708770357No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA1265580.24665692340830686No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA1263300.2462125597289101No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAAA1249960.24361264241173786No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAA1135840.22137107087982683No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA1122520.21877505148966686Illumina Paired End PCR Primer 2 (95% over 22bp)
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA1118220.21793699718203266Illumina PCR Primer Index 1 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA993750.1936782484212811No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA981610.191312206724844No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAATC956590.1864359000325165No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAAAAAAA955940.1863092174046183No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA947010.1845687929936477No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA926350.1805422343899912No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAA914160.17816644787602345No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAAA897340.17488829125871938Illumina PCR Primer Index 2 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA861660.16793438946886144No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAAA852250.1661004148095968No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA847250.16512593305653375No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA828670.16150475886215146No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAT799660.15585081573087964No Hit
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA764040.14890860772205847Illumina PCR Primer Index 2 (95% over 22bp)
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAA750700.14630869040488625No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA741460.14450784812522574No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA732530.14276742371425513No Hit
ATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAATCGTATGCCG724530.14120825290935424No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAAA715640.13947562435240815Illumina Paired End PCR Primer 2 (95% over 22bp)
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA711930.13875255889163537No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAAAAA697370.13591486802671576No Hit
AAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAAATCGTATGCC696950.13583301155945848No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA692270.13492089663859147No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAA673410.13124515146603766No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA656170.12788513838147625Illumina PCR Primer Index 1 (95% over 22bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAAAAA654610.12758110007452056No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA647420.1261797953136159No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAAAAAAAT645390.1257841557218723No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAA642530.12522675215912024No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAAA638270.12439649370551051No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAAA632420.12325635005442676Illumina PCR Primer Index 1 (95% over 22bp)
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA605490.11800779133242917Illumina PCR Primer Index 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAAA598040.11655581352036522No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAAAAAAAA588120.11462244172228814No Hit
AAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAAATCGTTTGCC579430.11292879243546457No Hit
AAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAAATCGTATGCCG576960.11244739844945141No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA556640.10848710460500317No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTACAAAAAAAAAAAAAAAAAA555240.10821424971414553No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA543840.10599243131716177Illumina PCR Primer Index 2 (95% over 22bp)
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA542160.10566500544813258No Hit
AAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAATCGTATGCCG516090.10058405758766184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)