FastQCFastQC Report
Fri 7 Jul 2023
SRR5933684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45884537
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA7038671.5339960823839194No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA4833691.0534463930626565No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAT3758560.8191343414885063No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA3411230.7434378165350127No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA3331720.7261095388191451No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA3291930.7174377721191781No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA3179420.6929175290577739No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA3114780.678829994514274No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA2954630.6439271687540402No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA2916680.6356564086066728No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA2239520.4880772797162582No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAA2050990.44698936375886283No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA1942650.42337792359112175No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA1874800.4085908069640105No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAATC1787950.38966286180462056No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA1518940.3310352679378676No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGAAAAAAAAAAAAAA1339250.2918739269397008No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA1299730.283261003592561No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA1283070.279630150784784No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA1277890.2785012301638785No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA1246300.2716165578831056No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1236570.26949601779789123No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAA1187610.2588257564852403No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1166130.2541444408603273No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAAA1128060.2458475281117035No Hit
ATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAAAATCGTATG1099300.23957962134389632No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1069590.23310467314947517No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAAA1041840.22705688410891017No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGAAAAAAAAAAAAAAAAA1016950.22163239873162502No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA900470.1962469404453182No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA875910.1908943747214884No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAAAAAAAAAAA874690.19062848994204737No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA849060.18504273019034714No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA760420.16572467539554775No Hit
AAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAAATCGTATGCC757150.1650120170113082No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA732950.15973790909124788Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA728830.1588400031147748No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAA714320.15567771774617667No Hit
ATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAATCGTATGCCG691070.1506106512527303No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAAAAA691050.15060629248585422Illumina Paired End PCR Primer 2 (95% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA677320.14761399902542333No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA669340.14587485104186623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAAA641360.13977693618222628No Hit
AAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAAATCGTTTGCC621600.13547047450865635No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCAAAAAAAAAAAAAAAAAAAATC618220.13473384290659837No Hit
AAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAATCGTATGCCG612910.13357659030099833No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAAAAAAAAAAA590220.12863156928008232No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA575080.12533198275488755No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA548150.11946290315624193No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAATCG547840.11939534226966265No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAAAAAA547810.11938880411934852No Hit
ATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAAATCGTATGC522910.11396213935862531No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA497700.10846791371132283No Hit
ACGGGGTCGGCGGCGATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTC496300.10816280002999704No Hit
ATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAATCGTTTGCCG474070.1033180306472309No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA461540.10058726319936495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)