FastQCFastQC Report
Tue 4 Jul 2023
SRR5933685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60018119
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA11625961.9370750356238258No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA10387481.7307240168589755No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA8009171.3344586823855642No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA6899601.1495861774675078No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA6768141.1276827919248853No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA6268691.0444662552653474No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA5066080.8440917650218263No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA5045330.8406344757322368No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA4779440.7963328540836143No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA4267510.7110369453597837No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA3493490.5820725571222917No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA3375240.562370173580415No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAT3280620.5466049344198874No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA2852290.47523815266519764No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA2769870.46150563299059744No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAAA2501850.4168491185136942No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA2342980.3903787787817875No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA2284240.38059173430610177No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA2047570.34115864244262634No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA1828630.30467965848779766No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1777350.296135571992851No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA1698510.2829995388559245No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAA1625600.27085154068223966No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA1517340.252813654489905No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1458430.24299828523449726No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAAA1445510.24084560197563004No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAAA1396610.23269806239679053No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAATC1369310.2281494360061501No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA1366920.2277512229265299No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA1289550.2148601158260225No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAAA1189410.19817515440628852No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA1166930.19442961882894066No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAAAAA1160800.19340826059543786No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA1101510.18352957712653406No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA1045600.1742140569250429No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA1045150.17413907956695543No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAATC1035560.1725412287579356No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA1017660.16955879606956692No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA1012180.16864573846441272No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA1006810.16775100865790213No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAAAAAATCGT974980.1624476101958477No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAA972110.1619694212676009No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA963500.16053485448286042No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAA884180.14731884549730723No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAAA875030.14579430588286182No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA857290.14283853181070202No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACAAAAAAAAAAAAAAAAAAA797160.13281989060670163No Hit
ATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAAAATCGTATG793250.13216842067309706No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA767380.12785805566482347No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAAAAAA728010.1212983699139255No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA716790.11942893445227765No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA712500.118714150305177No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA709790.11826261999313907No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAAT700270.11667643232871061No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAA700160.116658104530067No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA682110.11365067938900252No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA661860.11027669827506591No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAA636890.10611628798296727No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAAAAAAAT633620.10557145251419825No Hit
CGCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA630340.10502495088191618No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGGTAAAAAAAAAAAAAAAAAAA623050.103810317680899No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA610790.10176760121389343No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAA604720.10075623996146897No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA601660.1002463939264741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)