FastQCFastQC Report
Tue 4 Jul 2023
SRR5933686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60283314
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA9049261.5011218527236243No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA8326861.3812876976206054No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAT6393541.0605820376762962No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA5396390.8951714233892316No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA4981440.8263381140592237No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA4874540.8086051805313821No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA4109030.6816197928335526No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA3200000.5308268221617677No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAAA2880970.4779050468260587No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA2749140.45603664058681315No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAATC2653580.44018482461000735No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA2625730.4355649724233807No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA2391970.3967880730644636No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAAA2297770.3811618584870765No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAA1947240.32301475662071266No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAAA1913720.3174543456585681No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1792330.29731776192662535No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAAA1774890.2944247557458437No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAA1746500.28971532653297727No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAAAAAA1469000.2436826880486365No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA1441650.2391457775529726No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAAA1414300.23460886705730877No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAA1392950.2310672568531982No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAA1345360.22317286670736117No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1326040.21996799976855952No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAAAA1325800.21992818775689738No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGGAAAAAAAAAAAAAAAAAA1316790.21843357848574815No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAAA1310370.21736860717378612No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA1281650.21260443644488422No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1277520.21191933807753172No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAAAAAATCGT1269680.21061881236323537No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTTGAAAAAAAAAAAAAAAAAAA1237680.2053105441416177No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA1203750.1996821209928837No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA1190010.19740288332522662No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAAA1126670.18689582991406214No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAAAAA1126600.18688421807732733No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA1084030.17982256250875658No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA1076580.17858673131341118No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCAAAAAAAAAAAAAAAAAAAATC1032780.171321039185072No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA1017870.16884771796056203No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAAAAAAAAAAA941740.15621901609456973No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA922460.15302078449104506No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAAA917010.1521167200595508No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA889870.1476146450740913No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAAAAAAAAAAA877250.14552119679419084No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA874410.1450500879895223No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA867380.14388392781458564No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA859500.14257676676501227No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA857600.14226158833935376No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAA800040.13271334087571895No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAAAAAAATCG796920.13219578472411123No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA787010.130551880409229No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA785270.13026324332467853No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAAAAAAAAAAAAAAAA785250.13025992565704003No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAAA741910.12307053988438658No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA740720.12287313865989517No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAATCG737570.12235060600682968No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAT733090.12160744845580321No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAAA726850.12057233615258778No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAAAATC725780.12039484093392744No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAAAAA722060.11977775475316436No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAAAAAAAAAAAA693940.11511311405341784No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAATC693280.11500363102134696No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCCAAAAAAAAAAAAAAAAAAAAT685690.11374457615253201No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGAAAAAAAAAAAAAAAAA656720.10893893457814877No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA640660.1062748474644244No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA629930.10449491877636322No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA628730.10429585871805257No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAAA623340.10340174728947382No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCGAAAAAAAAAAAAAAAAAA622900.1033287586014266No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA622880.10332544093378808No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAAAT613130.10170807796001395No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA611070.10136635819324731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)