FastQCFastQC Report
Thu 6 Jul 2023
SRR5933688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26860146
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1175490.43763351100176445Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1007750.37518411106179395Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC918290.34187826082553685Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC820840.305597743214054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC819870.3052366133825185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC810940.30191198513961914Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC806530.3002701474519163Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC706480.26302165297240004Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC703850.2620425071405047Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC651430.24252660428576972Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC633730.23593691560723462Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC624150.2323702931473269Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC605420.22539713670953243Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC604610.22509557468526048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC603900.22483124254052828Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC597760.22254532793678783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC592620.22063171212844487Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC588840.21922442268184245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC588450.21907922615163744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC568460.21163697323164213Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC567230.2111790457133033Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC531380.1978321338983042Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC530920.1976608764524214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC521430.19412776088409944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC509320.18961922247183616Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC500530.18634671606029246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC492040.18318589928736798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC482550.17965278371904606Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC482540.17964906073109208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC470470.17515541427064468Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC466050.1735098535949879Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC458250.1706059229908877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC440520.16400506534849066Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC440520.16400506534849066Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC431870.1607846807683026Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC423190.1575531272242526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC419320.15611233088606444Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC412080.1534168876073868Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC407910.15186440163057938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC405430.15094110061799365Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC403260.15013321223198117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC402180.1497311295329519Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC371640.13836112432151337Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC365410.13604170282618716Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC362090.1348056708254676Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC356510.13272824354714974Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC342780.12761658108634258Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC338850.12615344682043053Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC333300.12408718850597461Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC333180.12404251265052692Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC333020.12398294484326333Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC327340.12186828768540572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC321870.11983181327458162Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC317060.11804105606871981Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC313420.11668588845347304Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC304420.11333519929489586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC282770.10527493037454078Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC275870.10270606868629827Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC273610.10186467340870002Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)