FastQCFastQC Report
Fri 7 Jul 2023
SRR5933689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53968954
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC770600.14278579495907961Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC614720.11390252255027956Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC592310.10975013523515761Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC572560.10609062387979579Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC555430.10291657681562626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC554070.10266458008432032Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)