FastQCFastQC Report
Fri 7 Jul 2023
SRR5933690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5933690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50483153
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC761630.15086815199518144Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC585750.11602880667932924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC550070.1089611023305141Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC530820.10514794905936245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC511450.10131102548210488Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)