FastQCFastQC Report
Sun 2 Jul 2023
SRR5938220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5938220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25454017
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC18410467.232830873020946No Hit
GCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGA2297810.902729812744291No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG1731560.680269837173441No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1318480.5179850394536941No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1313570.5160560708354992No Hit
TTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1201830.4721573023228514No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT1191040.46791828574641087No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1033760.40612843151633No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1021490.40130797429733783No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA999640.39272386751372096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC990950.3893098680652252No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC877450.3447196566262999No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG841140.330454717618834No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA836800.32874968222108125No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA806460.31683014904877294No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG747470.2936550250595024No Hit
TGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC716850.28162548960346806No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC695480.27322995816338147No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA683420.26849200265718376No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG674930.2651565762684923No Hit
ATCGCATGATGAGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA666820.261970438693429No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA656400.25787678227762634No Hit
TTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC655420.2574917742845854No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA649350.2551070819195257No Hit
GCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAAG572820.22504110058542037No Hit
ATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAA570290.2240471513788963No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG549360.21582448067037907No Hit
TTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA536870.21091759308560215No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA531600.20884719295975956No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA514680.202199912100318No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA512150.20120596289379394No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC503350.19774874826240588No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG492340.19342330132018062No Hit
ACTGACCCGGTGAGGCGGGGTTGTAGGCACCATCAATAGATCGGAAGAGC485580.19076753189879617No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG469950.1846270472750922No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC468180.18393167569582436No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA467460.18364881268052896No Hit
AGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG453500.1781644131061907No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGG445170.17489184516534265No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCGG442610.17388610999984794No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG439750.17276251524464684No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAA434220.17058997014105867No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC422190.1658638005938316No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGA407130.1599472491905698No Hit
GCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAGAG400220.15723254997433214No Hit
ATGTTTCCAAGAAAAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCA397430.15613645579006252No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG381750.14997632790140747No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG381660.14994097002449555No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG379560.14911595289655066No Hit
CTCGCTTCTGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG378000.14850308303007734No Hit
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT374410.14709269660659063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG369890.14531694545501403No Hit
GACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA367080.144212993964764No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA365940.14376512752387963No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCCTCAATAGATCGGAAGAGC364320.14312868573946502No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA361730.14211116461499967No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA354330.13920396140224153No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA346970.13631247280144426No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA336940.13237203385225993No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGG335920.13197131124725814No Hit
AAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA334800.13153130211235423No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA332400.13058842539470292No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC331760.13033699160332926No Hit
TAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAG326220.12816051784675087No Hit
ACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA323770.12719799786414854No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA321670.12637298073620365No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGA319590.1255558209142392No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGG318200.12500973814859948No Hit
TTAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAA318070.12495866565972673No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG316020.12415329179673291No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA315400.12390971531133965No Hit
CTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA308760.12130108972583777No Hit
ATCGCATGATGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT308400.1211596582181901No Hit
AGATCCCCGAATCCGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA307110.12065286198245251No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA305700.12009892191083239No Hit
AAGTCCTTCTGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT303310.11915997384617132No Hit
GCCGCCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG302280.11875532258817931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAG301980.11863746299847289No Hit
GTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC296380.11643741732395323No Hit
AACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATCGGAAGAG293670.11537275236360531No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG292000.11471666731423964No Hit
TTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC291240.11441808968698339No Hit
GTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA289010.11354200007016575No Hit
CTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA288600.11338092529756699No Hit
ACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG286920.11272091159521108No Hit
AAATGGGGGTAGAGCACTGAATCTGTAGGCACCATCAATAGATCGGAAGA284690.11184482197839343No Hit
TCTCGCTTCTGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC284270.11167981855280446No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG283880.11152660108618613No Hit
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT283850.11151481512721548No Hit
AGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATAGATCGGAA282220.11087444468981064No Hit
CGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAG281520.11059943898049569No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG277450.10900047721347872No Hit
TCGACTGCATAATTTGTGGTAGTCTGTAGGCACCATCAATAGATCGGAAG275090.1080733151077883No Hit
ACGTGGGGATGGAACACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA265350.10424680709532017No Hit
TATTCGAACGTCTGCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC259090.1017874703234464No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA256720.10085637956476574No Hit
TCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC256400.1007306626690789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)