FastQCFastQC Report
Sun 2 Jul 2023
SRR5938221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5938221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26034621
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC19543687.5068041128772345No Hit
GCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGA2388980.9176165844703481No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG1833270.7041661946989741No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1366950.5250508543988407No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1350760.5188322119227317No Hit
TTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1254760.4819582355356738No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT1219150.4682802949195996No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1161230.4460329958327413No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1065990.40945093842541436No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1029220.39532743726132985Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1019100.39144030558386084No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC903050.3468650455867977No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG881590.33862217544860745No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA876100.33651344492397256No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA838040.32189444970218695No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG789240.3031501783720992No Hit
TGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC773750.29720040864047914No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC715570.2748532425342393No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA702730.2699213481924703No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG697170.26778573039338655No Hit
ATCGCATGATGAGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA696000.26733632880616925No Hit
TTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC689600.26487806371369876No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA683270.26244668589567716No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA670880.2576876383182225No Hit
GCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAAG597190.22938302040194863No Hit
ATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAA592120.22743561352400712No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA580840.22310292129852785No Hit
TTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA576080.22127458663600288No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG568650.21842069450521287No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA532750.20463136375213606No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA532050.20436249100764708No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC524830.20158926070020378No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG508860.19545512108664842No Hit
ACTGACCCGGTGAGGCGGGGTTGTAGGCACCATCAATAGATCGGAAGAGC502480.19300453807259188No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG496890.19085739715588715No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC489060.18784986345681776No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA484260.18600616463746486No Hit
AGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG475740.18273359923311347No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGG471680.18117413731507748No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCGG456270.1752550958971133No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG454180.1744523187028534No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAA445330.1710529990046715No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC444540.1707495569073197No Hit
GCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAGAG416060.15981027724582586No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGA414660.1592725317568479No Hit
ATGTTTCCAAGAAAAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCA410450.15765545425070715No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG394610.1515712481468426No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG394030.15134846787283748No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG392550.15077999407020368No Hit
CTCGCTTCTGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG391090.15021920234598382No Hit
GACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA389840.1497390724451107No Hit
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT385820.14819497468390264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG380640.1462053163746843No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA377100.14484558849541157No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA368190.14142322256198775No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA366290.14069342511266056No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA362290.13915700942986647No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA361310.13878058758758194No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGG351900.13516616969380887No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA350080.13446710055813757No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC345120.1325619451114729No Hit
AAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA342140.1314173154277913No Hit
ACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA338730.13010752105820936No Hit
TAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAG336560.1292740155502936No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA330940.12711535151596792No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGA329330.1264969442036433No Hit
CTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA329050.1263893951058477No Hit
TTAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAA329040.12638555406664073No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG327580.12582476234242088No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGG327210.12568264389176245No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA326760.1255097971274481No Hit
ATCGCATGATGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT322300.1237966936411327No Hit
AGATCCCCGAATCCGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA317990.12214120574292209No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA317790.12206438495878238No Hit
AAGTCCTTCTGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT312740.12012466015925485No Hit
GCCGCCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG312180.1199095619636637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAG311350.11959075570948392No Hit
GTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC306340.11766639506678434No Hit
TTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC304110.11680984332362665No Hit
ACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG303040.11639885212847924No Hit
CTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA302670.11625673367782077No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG301820.11593024534522704No Hit
AACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATCGGAAGAG300590.11545779752276786No Hit
GTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA297810.11438998862322597No Hit
TCTCGCTTCTGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC296930.11405197717301127No Hit
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT295880.11364866805627784No Hit
AAATGGGGGTAGAGCACTGAATCTGTAGGCACCATCAATAGATCGGAAGA295760.11360257558579402No Hit
CGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAG291760.11206615990299992No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG291040.111789605080097No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCCTCAATAGATCGGAAGAGC290270.11149384506115914No Hit
AGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATAGATCGGAA287160.11029928186778673No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG285110.10951186883035478No Hit
TCGACTGCATAATTTGTGGTAGTCTGTAGGCACCATCAATAGATCGGAAG283390.10885121008675333No Hit
ACGTGGGGATGGAACACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA272480.10466063631193248No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA267900.10290144035513327No Hit
TAATCAAACTCCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC264580.10162621533841418No Hit
TATTCGAACGTCTGCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC264500.1015954870247583No Hit
CCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC263930.10137654778996014No Hit
ACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC262620.10087337165384509No Hit
TCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC262390.10078502775208442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)