FastQCFastQC Report
Tue 4 Jul 2023
SRR5938222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5938222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36874811
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8273752.243740313679167Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC4384971.1891505016798594No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT4220411.144523832271303No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG3554220.9638612113835647No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2818980.76447307079079No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2558050.6937120301443714No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC2493470.6761987200422532No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1884990.5111863488602016No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1596070.43283476083443523No Hit
TTAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAA1453690.39422303750926346No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA1277710.3464994030749066No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG1170390.3173955250916405No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG1169550.31716772731391085No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA1160280.31465381612396603No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA1102150.2988896675294146No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1052470.28541705610369095No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG934690.2534765534120297No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGA932350.2528419738883543No Hit
TAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAG900680.24425345529228612No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAA881990.23918495473780188No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG878750.23830630616655907No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG876600.2377232523307035No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA836010.22671573828541114No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGG830240.2251509845026731No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC797630.21630754934581226No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA792670.21496245770588493No Hit
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT790490.21437126823511043No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG784930.21286346389680477No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA776760.21064785932055352No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA746120.2023386641900348No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA744590.20192374680917008No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGA741310.201034250724702No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG732790.1987237304077301No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA723850.19629931120189334No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA714440.19374743371566025No Hit
GTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA700020.18983690519796834No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT693660.18811215059515832No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG689400.18695689043667235No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG669970.18168771088752156No Hit
GTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC663930.18004973639051328No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA638670.17319953178878666No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA635980.17247003652439058No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA630100.1708754520802832No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG620570.16829103205437446No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGG608330.16497169300745704No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT599910.16268829147354816No Hit
TCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC586820.1591384427705948No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG534630.1449851498899886No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA531500.1441363319801151No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA525160.14241700113391767No Hit
TCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG512740.13904884827748676No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG509570.13818918285438805No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG508800.13798036822480256No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGG502240.13620137605586644No Hit
ACGTGGGGATGGAACACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA488920.13258915415186806No Hit
CCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC485800.13174304812030088No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCAATAGATC474730.12874099883522114No Hit
GCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGA471940.12798438478776203No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC458820.12442640044988977No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA458320.12429080653457451No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG457980.12419860267216014No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA456410.12377283777807023No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA454250.12318707206390835No Hit
TCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG441150.11963451148264868No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA439750.11925484851976598No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCGG436240.1183029792342529No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC431690.11706907460488408No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA431030.11689009063666794No Hit
TGAATGATTTCATGTTTCCAAGAAAAGCTTCTGTAGGCACCATCAATAGA428290.11614703598074035No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG422450.11456329904985818No Hit
TCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA404770.10976869820431079No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA403360.10938632336312178No Hit
GACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA401110.10877615074420313No Hit
CGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC396270.10746360164395148No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG373490.1012859428621885No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA372030.10089000862946795No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC369330.10015780148676559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)