FastQCFastQC Report
Sun 2 Jul 2023
SRR5938223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5938223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37205981
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8231262.212348600618809Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC4401571.183027535277191No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT4235281.1383331083247072No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG3579900.9621840101461107No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2819530.7578163306593099No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2614610.7027391644370296No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC2526050.6789365397998779No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1911640.5137991120298643No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1571410.42235413709424835No Hit
TTAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAA1463160.393259352575598No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA1275950.3429421737327662No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG1183570.318112832450245No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG1177490.3164786865853638No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA1164980.31311632395877426No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA1109210.29812679848436197No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1042230.2801243165715749No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGA938980.25237340200759656No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG937190.251892296563824No Hit
TAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAG903050.24271635251332307No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAA885750.2380665624701577No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG884460.23771984402185228No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG883580.23748332290983No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA844310.22692856828583555No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGG835430.22454185524633796No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC803200.21587926951852177No Hit
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT801550.21543579243347996No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA800320.21510520042463066No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA777400.20894490055241385No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG775060.20831596941362734No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA751760.2020535354248555No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA746210.20056183977516948No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGA745140.20027425160486964No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG742170.19947599285179443No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA729420.19604912446738068No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA720240.1935817792306027No Hit
GTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA708800.19050700477431304No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT698820.18782464034478757No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG693460.18638401175337913No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG670930.18032853373762675No Hit
GTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC666420.17911636303851255No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA636300.17102089043156798No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA634000.17040271025241882No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG630200.16938136908686807No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA624640.1678869856972727No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGG610800.1641671536627404No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT605040.1626190154749582No Hit
TCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC591190.15889649570051653No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA532770.14319471915012805No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG532230.14304958119502345No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA529870.14241527457641823No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG513630.13805038496364333No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG508200.13659094219286946No Hit
TCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG507970.13652912417495455No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGG502110.13495410858807888No Hit
ACGTGGGGATGGAACACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA489900.13167237815876967No Hit
CCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC488580.13131759649073627No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCAATAGATC479390.128847563514049No Hit
GCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGA473860.1273612433441817No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA465040.12499065674414014No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG460550.12378386152484462No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA458540.12324362580306644No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA456920.12280821193775268No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC454770.1222303478572437No Hit
TCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG442520.11893786646829713No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA439110.11802134715921077No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA435500.11705107305193753No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC434840.11687368221792083No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCGG434250.11671510556326953No Hit
TGAATGATTTCATGTTTCCAAGAAAAGCTTCTGTAGGCACCATCAATAGA429670.1154841206847899No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG427670.11494657270292107No Hit
GACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA409560.11007907572709882No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA407090.10941520396949081No Hit
TCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA405610.1090174184629079No Hit
CGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC391550.10523845615037003No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG379760.10206961079725328No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)