FastQCFastQC Report
Tue 4 Jul 2023
SRR5938225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5938225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34260531
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT2500720.7299127967397819No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA2301790.671848898080418No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC2127310.6209214912635184No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2094730.6114120064280382No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1677680.48968301162641054No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGG1660010.48452547335007745No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1279260.3733917609157897Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA1272860.3715237221513No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG1176010.34325504178554617No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG1018810.2973713396327687No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1008140.29425696875509605No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC966310.28204758414281433No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAG959970.2801970582417418No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG928960.27114582666567544No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC921620.26900341970765135No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG901510.26313369165235645No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA892560.26052135619264044No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA869740.253860630473007No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG866360.25287407250051086No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT844560.24651106545896792No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT793530.23161637512273234No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA772990.22562113821294832No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGG765300.2233765728849912No Hit
CCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC748310.21841751372738502No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC744200.21721788258331431No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC743560.21703107870686533No Hit
AGGATAGCTGGCGCTCTCGCAGACCCTGTAGGCACCATCAATAGATCGGA743530.21702232227515678No Hit
TCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC722780.21096579034341295No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT688740.20103015916478353No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGA685500.20008446454026063No Hit
GTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC673910.1967015630901926No Hit
CGCGACCTCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT648050.18915351895742655No Hit
GTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA632510.18461768733240008No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA623690.18204329641008776No Hit
AGTGCGGTAACGCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA601470.17555769932462517No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG597220.17431720483258126No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA573260.16732373470802306No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGA571780.16689175074373483No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA561360.16385035013030008No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG554730.16191517872271155No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC547350.1597610965224094No Hit
TATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAA543980.15877745736048282No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA538740.1572480006220569No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCAATAGATC537520.15689190573257605No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG531840.15523402132909148No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAA516820.1508499678536798No Hit
TAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA509430.1486929668428081No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA501820.14647175199940715No Hit
TCAAACTTTAAATGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA496380.14488391904959091No Hit
AGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT491280.1433953256591382No Hit
ACGTGGGGATGGAACACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA481600.14056991702784757No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG470470.13732128086397727No Hit
AACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC470300.1372716610842955No Hit
GACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA470280.13726582346315647No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG468060.13661784751672412No Hit
AGATCCCCGAATCCGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA454000.1325139998559859No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG448490.13090573523218305No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG446570.13034532360283616No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG445680.1300855494621493No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA443440.12943173589457793No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCTGTAGGCACCAT443390.12941714184173037No Hit
ACGTGGGGATGGAACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC440610.12860571250340516No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA431370.12590873153717322No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG430270.12558766237452654No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT428730.1251381655468212No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA427760.12485504092157826No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA416670.12161807999998599No Hit
CGCGCCGGGCCGTACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA415590.12130284845847837No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA412510.1204038548030677No Hit
CTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC404950.11819723401251428No Hit
ATACCCGGCCGTCGCCGGCAGTCGAGACTGTAGGCACCATCAATAGATCG404270.11799875489378725No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCA404190.11797540440923113No Hit
GTCTACGGCCATACCACCCTGAACCTGTAGGCACCATCAATAGATCGGAA404070.11794037868239696No Hit
GTCTACGGCCATACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG398830.11641092194397103No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC395810.11552944115197747No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG389820.11378107362083793No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC380760.11113663124485723No Hit
TAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATAGATCG372490.10872277490386825No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG370530.10815068803224329No Hit
ATTGATCATCGACACTTCGAACGCCTGTAGGCACCATCAATAGATCGGAA365210.10659788080926126No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC363280.10603455036934484No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA356790.10414024230972954No Hit
GTCACGCACCGCACGTTCGCTGTAGGCACCATCAATAGATCGGAAGAGCA354420.10344848420475446No Hit
CGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCA352790.10297271808192349No Hit
CGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC352510.10289099138597706No Hit
TGGAATGAGTCCACTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA349620.10204745513138719No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA347500.10142866729064999No Hit
ACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG345600.10087409328244212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)