FastQCFastQC Report
Tue 27 Jun 2023
SRR594900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30900888
Total Bases618 Mbp
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATTCAATGAAGCGGAG6662732.156161337499427No Hit
TAGCCTGTGGGAATACTGCC6062971.962069827896208No Hit
TCGGGGCTGATCCGGGTTGA5164101.6711817472688812No Hit
AGCGGAGAATTTGGACAAAC4977441.6107757162188996No Hit
ACTGCCAGCTGGGACTGAGG4718891.527104981578523No Hit
CGGGGCTGATCCGGGTTGAA4413911.4284087887700831No Hit
TGTGGCGTCGCTGAACCATA4165921.3481554316497313No Hit
GTTTTCATGCCAGGTCGTAC4082111.3210332337374902No Hit
ATTCAATGAAGCGGAGATCT3873311.253462360046093No Hit
TGGGACTGAGGACTGCGACG3696171.1961371466088613No Hit
GTGGCGTCGCTGAACCATAG3688881.1937779911049806No Hit
CTGCCAGCTGGGACTGAGGA3492571.13024907245384No Hit
AATAGGGACGGTCGGGGGCA3013660.9752664713065851No Hit
AATGATTAATAGGGACGGTC2724270.8816154409543182No Hit
GTATGCCGTCTTCTGCTTGA2411120.7802753111819958No Hit
AGCTGGGACTGAGGACTGCG2411080.7802623665701776No Hit
TAATGATTAATAGGGACGGT2323500.7519201389940638No Hit
AGGGACGGTCGGGGGCATCT1933200.625613089177243No Hit
TACTGCCAGCTGGGACTGAG1864260.6033030507084457No Hit
GTTGAAAGGGAAGGGCATTT1815700.5875882919610596No Hit
ATAGCCTGTGGGAATACTGC1752670.5671908198884122No Hit
TGCCAGCTGGGACTGAGGAC1729120.5595696796804027No Hit
AATGAAGCGGAGATCTCGTA1603460.518904181653291No Hit
TTGCTCGAATATATTAGCAT1436990.4650319434185839No Hit
AGGGACGGTCGGGGGCATCA1370230.4434273862938825No Hit
GGGACTGAGGACTGCGACGA1342960.4346023971867734No Hit
TCGAAGAGTCGAGTTGTTTG1171140.37899881712137207No Hit
TAGAATAATGTAGATAAGGG1080320.3496080759879781No Hit
CTGGGACTGAGGACTGCGAC1019180.32982223682374434No Hit
GTAATGATTAATAGGGACGG1015810.32873165327805465No Hit
GAGCGGAGAATTTGGACAAA1005950.3255408064648498No Hit
GCCAGCTGGGACTGAGGACT944210.30556079812334197No Hit
AGGGACGGTCGGGGGCATAT942700.30507213902720204No Hit
GTTGAAAGGGAAGGGCATCT939910.30416925235287734No Hit
GCGGAGAATTTGGACAAACT925310.29944446903920685No Hit
ATCTCGTATGCCGTCTTCTG922790.29862895849465554No Hit
AAGTGGAGAAAGAAGAGAAC862280.27904699696655966No Hit
CTACATGGTATAACTGTGGT841920.27245818955105755No Hit
GTTTCTTTACTTATTCAATG828090.2679825900148889No Hit
AACGGGGTATTGTAAGCATC799950.25887605560073224No Hit
ATTAGCATGGAATAATAGAA797200.2579861135382258No Hit
AATGACCCACTCGGCACCTT769480.2490154975481611No Hit
AATAGTAATTGAACTTAGTA742190.24018403613514278No Hit
TTGTTGAAAGGGAAGGGCAT740040.2394882632499105No Hit
ATTGTGAAGAGACATAGAGG739950.23945913787331938No Hit
TTTGATGATTCATAATAACT721740.23356610334304956No Hit
AAATGAGAACTTTGAAGACT711600.23028464424711675No Hit
TTGTGGCGTCGCTGAACCAT675030.21845003289225864No Hit
GGTCTGTTGAGTGCAATCGT640480.20726912443422338No Hit
TTAACGATCAACTTAGAACT636960.20612999859421516No Hit
TAACGATCAACTTAGAACTG632130.20456693671715842No Hit
GCTGGGACTGAGGACTGCGA619960.20062853857144816No Hit
TACATGGTATAACTGTGGTA600270.1942565534039022No Hit
GGGGCTGATCCGGGTTGAAG597730.1934345705534417No Hit
ATGAGGTAAAGCGAATGATT597070.19322098445844016No Hit
GTTCTACAGTCCGACGATCG588240.1903634613995559No Hit
ACTAGAAGACTTAAGAGAAA585960.1896256185259142No Hit
TTATTCAATGAAGCGGAGAT580330.18780366441249197No Hit
AGCCTGTGGGAATACTGCCA564010.18252226279063566No Hit
AGCGGAGAATTTGGACATCT556170.17998511887425372No Hit
GAGGACTGCGACGATCTCGT547130.17705963660332352No Hit
ATGATTAATAGGGACGGTCG543190.1757845923392234No Hit
ATAGAATAATGTAGATAAGG540580.17493995641808094No Hit
ACTTATTCAATGAAGCGGAA539280.17451925653398698No Hit
GTAACGGCGAGTGAAGCGGC528260.17095301597805215No Hit
TGAGGACTGCGACGATCTCG505320.1635292811002713No Hit
AAGGATTGGCTCTAAGGGTC495670.16040639349911237No Hit
AAGGCTGAAACTTAAAGGAA489790.15850353556182592No Hit
GAGATTAAGCCTTTGTTGTC484420.15676572142522247No Hit
AGCGGAGATCTCGTATGCCG482640.15618968619930923No Hit
CAGCTGGGACTGAGGACTGC481190.15572044402089674No Hit
ATACTGCCAGCTGGGACTGA479410.1551444087949835No Hit
ATAGAGGGTGTAGAATAAGT459350.14865268596811845No Hit
TTCAGGGAGGTAGTGACAAT459160.14859119906198165No Hit
ATTCAGGGAGGTAGTGACAA454170.1469763587376518No Hit
AACGATCAACTTAGAACTGG448580.14516734923604785No Hit
AACGGGGTATTGTAAGCAGT439330.14217390775307168No Hit
ATTGTTGAAAGGGAAGGGCA430140.13919988318782295No Hit
ATTCAATGAAGCGGAGCTGG420520.13608670404552775No Hit
CAGTTTTGGTGGCAGGATAA417450.13509320508847514No Hit
ATCCTGCCAGTAGTCGTATG415930.13460130983938065No Hit
AGTTTCTTTACTTATTCAAT414680.13419679072005955No Hit
ATGGCTGGAAGAGGCATCTC413090.13368224240028312No Hit
AACTTAGAACTGGTACGGAC403140.13046227021048715No Hit
AACTAGAAGACTTAAGAGAA399030.12913221134615938No Hit
GGGACTGAGGACTGCGACGT396520.1283199369545626No Hit
CCAGCTGGGACTGAGGACTG389740.12612582525136495No Hit
AGGGACGGTCGGGGGCAATC370690.11996095387291135No Hit
AAGGGAAGTCGGCATCTCGT367180.1188250641858577No Hit
TTGAAAGGGAAGGGCATTTG354750.11480252606332866No Hit
GTTACGATCTGCTGAGATTA354400.11468926070991876No Hit
TTCGGGGCTGATCCGGGTTG351990.11390934784786767No Hit
TTTCTTTACTTATTCAATGA349090.11297086349104271No Hit
ATGGAGAACAGAAATCTCCA335340.10852115317851059No Hit
AAGAGGTGCACAATCGACCG330420.10692896592486274No Hit
ATTCAATGAAGCGGAGCATC327830.10609080230962942No Hit
CAATGAAGCGGAGATCTCGT322750.10444683660870847No Hit
ATTGCGATGGTCAGAAAGTG309540.10017187855572306No Hit
AATGTAGATAAGGGAAGTCG309230.10007155781413142No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (21 vs 0)

Produced by FastQC (version 0.12.1)