FastQCFastQC Report
Tue 27 Jun 2023
SRR594901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30900888
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTG5381531.741545420960071RNA PCR Primer, Index 47 (96% over 25bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCG5166071.671819269400931No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCATCTCGTATG3875121.25404810373087No Hit
ATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTG3854561.2473945732562766RNA PCR Primer, Index 47 (96% over 29bp)
GTTTTCATGCCAGGTCGTACTGATAACCGCATCTCGTATG3807311.232103750545939No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGATCTCGTATGC3002030.9715028254204215No Hit
CGGGGCTGATCCGGGTTGAAGACATTGTCATCTCGTATGC2854610.9237954585641681No Hit
TGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCTG2741630.8872334024834496RNA PCR Primer, Index 47 (96% over 25bp)
CTGCCAGCTGGGACTGAGGACTGCGACGATCTCGTATGCC2272070.7352766043487164No Hit
GTGGCGTCGCTGAACCATAGCAGGCTAGCATCTCGTATGC2235770.7235293691236316No Hit
AGGGACGGTCGGGGGCATCTCGTATGCCGTCTTCTGCTTG1900450.6150146882510302Illumina PCR Primer Index 3 (96% over 28bp)
AGCGGAGAATTTGGACAAACTTGGTCATTATCTCGTATGC1814470.5871902451476475No Hit
AATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAA1594730.5160790201239525RNA PCR Primer, Index 47 (96% over 29bp)
AGCTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTT1542100.49904714712405673Illumina PCR Primer Index 1 (95% over 21bp)
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGC1431020.4630999601047064No Hit
AATAGGGACGGTCGGGGGCATCTCGTATGCCGTCTTCTGC1421810.46011946323354846Illumina PCR Primer Index 3 (96% over 25bp)
TACTGCCAGCTGGGACTGAGGACTGCGACGATCTCGTATG1320020.42717866230899254No Hit
GGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCTGC1298400.4201820996212148Illumina PCR Primer Index 1 (96% over 25bp)
TGTGGCGTCGCTGAACCATAGCAGGCTAGCATCTCGTATG1260820.40802063681794515No Hit
TGCCAGCTGGGACTGAGGACTGCGACGATCTCGTATGCCG1123150.3634685190923963No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATATCTCGT1061930.34365679070452604No Hit
TGTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTTC1018820.3297057353173799RNA PCR Primer, Index 24 (95% over 23bp)
TAATGATTAATAGGGACGGTCGGGGGCATCTCGTATGCCG999380.3234146539736981No Hit
GTTGAAAGGGAAGGGCATCTCGTATGCCGTCTTCTGCTTG933980.30225021365081806Illumina PCR Primer Index 3 (96% over 28bp)
AATGATTAATAGGGACGGTCGGGGGCATCATCTCGTATGC929080.3006644987030794No Hit
AGGGACGGTCGGGGGCATATCTCGTATGCCGTCTTCTGCT927130.30003344887693845RNA PCR Primer, Index 37 (96% over 27bp)
AGGGACGGTCGGGGGCATCATCTCGTATGCCGTCTTCTGC905850.293146915389616Illumina PCR Primer Index 7 (96% over 25bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTATCTCGTATG875920.28346110959659154No Hit
AATGATTAATAGGGACGGTCGGGGGCATCTCGTATGCCGT813020.2631057075123537No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTATCTCGTATG805530.26068182894938163No Hit
AACGGGGTATTGTAAGCATCTCGTATGCCGTCTTCTGCTT794430.2570896991698103RNA PCR Primer, Index 43 (96% over 27bp)
CTGCCAGCTGGGACTGAGGACTGCGACGTATCTCGTATGC769770.24910934598384357No Hit
CTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCT739970.2394656101792285Illumina PCR Primer Index 1 (95% over 23bp)
GTAATGATTAATAGGGACGGTCGGGGGCATCTCGTATGCC734690.23775692141921617No Hit
GTTGAAAGGGAAGGGCATTTGATCAGACATCTCGTATGCC722580.23383794019123336No Hit
TGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCTTCT719150.23272793972781625RNA PCR Primer, Index 2 (96% over 25bp)
ACTTATTCAATGAAGCGGAGCATCTCGTATGCCGTCTTCT708990.2294400083259743RNA PCR Primer, Index 24 (95% over 24bp)
CGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCTT695000.22491263034253253RNA PCR Primer, Index 41 (95% over 23bp)
ATTAGCATGGAATAATAGAATATCTCGTATGCCGTCTTCT690100.22332691539479382RNA PCR Primer, Index 37 (95% over 24bp)
AATAGTAATTGAACTTAGTACGAGAGGAACATCTCGTATG688290.22274117171001687No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATGC642780.2080134396137742No Hit
GGTCTGTTGAGTGCAATCGTATGCCGTCTTCTGCTTGAAA632810.2047869951180691Illumina Single End Adapter 1 (95% over 22bp)
AATGACCCACTCGGCACCTTACGAGAAATCATCTCGTATG595400.19268054691502717No Hit
TTATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTGCT566360.18328275873495933RNA PCR Primer, Index 47 (96% over 27bp)
TCGAAGAGTCGAGTTGTTTGGGAATGCATCTCGTATGCCG566150.1832147995229134No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGATCTCGTATGC564410.1826517089088184No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCATCTCGTATG563410.18232809361336152No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGATCTCGTATGCC560440.1813669561858546No Hit
AGCGGAGAATTTGGACATCTCGTATGCCGTCTTCTGCTTG553190.1790207452937922RNA PCR Primer, Index 41 (96% over 28bp)
GAGGACTGCGACGATCTCGTATGCCGTCTTCTGCTTGAAA543570.175907566151497RNA PCR Primer, Index 47 (96% over 29bp)
AGCGGAGAATTTGGACAAACTTGGTCATATCTCGTATGCC534580.17299826464533963No Hit
AATAGGGACGGTCGGGGGCATCATCTCGTATGCCGTCTTC528440.17101126673123437Illumina PCR Primer Index 7 (95% over 22bp)
ATTGTGAAGAGACATAGAGGGTGTAGAATATCTCGTATGC522920.16922491030031242No Hit
GTTGAAAGGGAAGGGCATTTGATCAGACAATCTCGTATGC518940.167936921424394No Hit
ACTTATTCAATGAAGCGGAATCTCGTATGCCGTCTTCTGC518600.16782689222393868Illumina PCR Primer Index 3 (95% over 24bp)
GCCAGCTGGGACTGAGGACTGCGACGATCTCGTATGCCGT517150.16735765004552622No Hit
AATGATTAATAGGGACGGTCGGGGGCATATCTCGTATGCC508910.16469106001096148No Hit
TGAGGACTGCGACGATCTCGTATGCCGTCTTCTGCTTGAA501730.1623675021895811Illumina PCR Primer Index 1 (96% over 28bp)
AAGTGGAGAAAGAAGAGAACTAGAAGACATCTCGTATGCC484730.1568660421668141No Hit
AGCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA479690.15523502107771142RNA PCR Primer, Index 47 (96% over 29bp)
ACTAGAAGACTTAAGAGAAAGAGACGGAATCTCGTATGCC462510.1496753103017622No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGA456840.1478404115765217No Hit
AATAGGGACGGTCGGGGGCATATCTCGTATGCCGTCTTCT445520.1441770864319498RNA PCR Primer, Index 37 (95% over 24bp)
TAGAATAATGTAGATAAGGGAAGTCGGCATCTCGTATGCC445470.14416090566717693No Hit
GCTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTC436540.14127102107874698RNA PCR Primer, Index 47 (95% over 23bp)
AGCTGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCT428280.13859795873827316No Hit
ATCCTGCCAGTAGTCGTATGCCGTCTTCTGCTTGAAAAAA414440.1341191230491499Illumina Single End Adapter 1 (95% over 22bp)
ATGGCTGGAAGAGGCATCTCGTATGCCGTCTTCTGCTTGA404430.13087973394162652Illumina PCR Primer Index 3 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCGT402230.1301677802916214No Hit
TGCCAGCTGGGACTGAGGACTGCGACGTATCTCGTATGCC391640.12674069431273302No Hit
TTTGATGATTCATAATAACTTTTCGAATCGTATGCCGTCT376760.12192529871633462No Hit
AGGGACGGTCGGGGGCAATCTCGTATGCCGTCTTCTGCTT366370.11856293579653762RNA PCR Primer, Index 48 (96% over 28bp)
AAGGGAAGTCGGCATCTCGTATGCCGTCTTCTGCTTGAAA364750.11803867901789748TruSeq Adapter, Index 18 (96% over 30bp)
GGGACTGAGGACTGCGACGTATCTCGTATGCCGTCTTCTG360550.11667949477697857TruSeq Adapter, Index 2 (96% over 26bp)
TAATGATTAATAGGGACGGTCGGGGGCATATCTCGTATGC359740.11641736638765851No Hit
GTTCTACAGTCCGACGATCGTATGCCGTCTTCTGCTTGAA342950.11098386557693747Illumina Single End Adapter 1 (95% over 23bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGATCTCGTAT342200.11074115410534481No Hit
GTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTTCT340240.11010686812624933RNA PCR Primer, Index 34 (96% over 27bp)
ATTCAGGGAGGTAGTGACAATAAATAACGATCTCGTATGC332040.10745322270350288No Hit
ATTCAATGAAGCGGAGCATCTCGTATGCCGTCTTCTGCTT323170.10458275503280035RNA PCR Primer, Index 26 (100% over 27bp)
ATAGAGGGTGTAGAATAAGTGGGAGCTTATCTCGTATGCC321810.104142638230979No Hit
CAATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTGAAA320520.10372517449983962RNA PCR Primer, Index 47 (96% over 29bp)
TAGAATAATGTAGATAAGGGAAGTCGGCAATCTCGTATGC316700.10248896407119433No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA309620.1001977677793596Illumina Paired End PCR Primer 2 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)