FastQCFastQC Report
Tue 27 Jun 2023
SRR594902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41484491
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA8394762.0235899724550075Illumina Paired End PCR Primer 2 (100% over 24bp)
TCGGGGCTGATCCGGGTTGAAGACATATCTCGTATGCCGT5405441.3030026088544753No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCATCTCGTATG5384391.2979284234197304No Hit
GTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA4750681.1451701311702245No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATGC3529700.8508480916398371No Hit
ACTTATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTG2658990.6409600156357227RNA PCR Primer, Index 47 (96% over 25bp)
TCGGGGCTGATCCGGGTTGAAGACATTATCTCGTATGCCG2601070.6269981714371282No Hit
TTCGGGGCTGATCCGGGTTGAAGACATATCTCGTATGCCG2576110.6209814650974023No Hit
CGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCTT2402330.5790911114228205RNA PCR Primer, Index 41 (95% over 23bp)
TGTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTTC2265380.5460787743544931RNA PCR Primer, Index 24 (95% over 23bp)
CGGGGCTGATCCGGGTTGAAGACATTGTCATCTCGTATGC2088340.5034025848358608No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCATCTCGTATGCCG1976410.47642141734365256No Hit
TTCGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTC1927480.4646266480646948RNA PCR Primer, Index 41 (95% over 21bp)
TCGGGGCTGATCCGGGTTGAAGACAATCTCGTATGCCGTC1888120.4551387649905117No Hit
GTGAAATTGTTGAAAGGGAAGGGCATCTCGTATGCCGTCT1824250.43974264984955463RNA PCR Primer, Index 34 (95% over 21bp)
ATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTG1496760.36079989507404103RNA PCR Primer, Index 47 (96% over 29bp)
CGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATGCC1488770.35887387409429705No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCG1441710.34752987568293897No Hit
ATGAGGTAAAGCGAATGATTAGAGGTATCTCGTATGCCGT1404870.3386494485372859No Hit
AGCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCGT1398730.3371693773463437No Hit
AAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCAC1319790.31814057933120116No Hit
TTCGGGGCTGATCCGGGTTGAAGACAATCTCGTATGCCGT1304330.3144138854204575No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTA1278370.3081561251408388No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1262880.30442219961189837No Hit
ATTGTGAAGAGACATAGAGGGTGTAGAATATCTCGTATGC1244700.3000398389846461No Hit
ACGTGAAATTGTTGAAAGGGAAGGGCATCTCGTATGCCGT1235070.29771848954347785No Hit
TGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCTG1234560.29759555203413246RNA PCR Primer, Index 47 (96% over 25bp)
TTGTGGCGTCGCTGAACCATAGCAGGCATCTCGTATGCCG1194940.2880449949355773No Hit
AAATGAGAACTTTGAAGACTGAAGTATCTCGTATGCCGTC1136290.27390718136086084No Hit
GAGCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCG1075660.2592920809851566No Hit
AAATGAGAACTTTGAAGACTATCTCGTATGCCGTCTTCTG772930.1863178217613903Illumina PCR Primer Index 1 (95% over 23bp)
CTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCT767800.1850812150497399Illumina PCR Primer Index 1 (95% over 23bp)
TGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCTTCT756380.18232837905616342RNA PCR Primer, Index 2 (96% over 25bp)
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGC740230.1784353579268937No Hit
TCGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCT724600.17466768484636824No Hit
AAATGAGAACTTTGAAGACTGAATCTCGTATGCCGTCTTC716510.17271755847263498TruSeq Adapter, Index 8 (95% over 22bp)
GTTTTCATGCCAGGTCGTACTGATAACCGCATCTCGTATG679290.16374553082982266No Hit
AGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAA658530.1587412510376468No Hit
TTCGGGGCTGATCCGGGTTGAAGACATTATCTCGTATGCC648020.15620777412937284No Hit
AACTTAGAACTGGTACGGACATCTCGTATGCCGTCTTCTG647920.15618366873538353RNA PCR Primer, Index 20 (95% over 24bp)
TGAACTCTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAA646910.15594020425609176Illumina Single End Adapter 1 (96% over 25bp)
TTCGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATG640610.15442156443476673No Hit
ATTGTGAAGAGACATAGAGGGTGTAGAATCTCGTATGCCG624850.1506225543420552No Hit
TTGTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTT581360.1401391184961146RNA PCR Primer, Index 34 (96% over 25bp)
AGCTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTT575840.138808500747906Illumina PCR Primer Index 1 (95% over 21bp)
AACGGGGTATTGTAAGCATCTCGTATGCCGTCTTCTGCTT575150.13864217352937994RNA PCR Primer, Index 43 (96% over 27bp)
AATAGTAATTGAACTTAGTACGAGAGGAACATCTCGTATG559650.13490583746104057No Hit
AGCTGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCT543110.1309188052952126No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTATCTCGTATG542860.1308585418102394No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGATCTCGTATGCCGT535280.1290313529458515No Hit
AACTCTGGTGGAGGCTCGTATGCCGTCTTCTGCTTGAAAA516840.12458631829422712TruSeq Adapter, Index 15 (96% over 25bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGATCTCGTATGC511510.12330150079459816No Hit
AATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAA508390.12254941250213242RNA PCR Primer, Index 47 (96% over 29bp)
GTTGAAAGGGAAGGGCATCTCGTATGCCGTCTTCTGCTTG499160.1203244846369213Illumina PCR Primer Index 3 (96% over 28bp)
AAGGATTGGCTCTAAGGGTCGGATCTCGTATGCCGTCTTC496400.119659175762817TruSeq Adapter, Index 9 (95% over 22bp)
GTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTTCT492660.11875763402761769RNA PCR Primer, Index 34 (96% over 27bp)
TCTGAACTCTGAACTCGTATGCCGTCTTCTGCTTGAAAAA484640.11682438142967694Illumina Single End Adapter 1 (96% over 25bp)
TAGAATAATGTAGATAAGGGAAGTCGGCATCTCGTATGCC482830.11638807379847084No Hit
ATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGT470340.11337731008920901No Hit
AACTGAATGTGGAGACGTATCTCGTATGCCGTCTTCTGCT462630.11151878421263503Illumina PCR Primer Index 2 (96% over 27bp)
TGTGGCGTCGCTGAACCATAGCAGGCATCTCGTATGCCGT456130.10995193360333141No Hit
GGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCTTC455460.10979042746360321RNA PCR Primer, Index 41 (95% over 24bp)
AATGATTAATAGGGACGGTCGGGGGCATCTCGTATGCCGT441710.10647593579007635No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC441210.1063554088201299Illumina Paired End PCR Primer 2 (100% over 24bp)
CTGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCTTC435720.1050320226901181TruSeq Adapter, Index 2 (95% over 24bp)
AAATGAGAACTTTGAAGACTGATCTCGTATGCCGTCTTCT427700.10309877009217734Illumina Paired End PCR Primer 2 (96% over 25bp)
GCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCGTC427190.10297583258283198No Hit
CGGGGCTGATCCGGGTTGAAGACATATCTCGTATGCCGTC425070.10246479823025911No Hit
CGGGGCTGATCCGGGTTGAAGACATTATCTCGTATGCCGT421140.10151745624648018No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA415970.10027120737723406Illumina Paired End PCR Primer 2 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)