FastQCFastQC Report
Tue 27 Jun 2023
SRR594903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41183182
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA8783172.1327079583117206Illumina Paired End PCR Primer 2 (100% over 24bp)
TCGGGGCTGATCCGGGTTGAAGACATATCTCGTATGCCGT5414801.314808554618242No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCATCTCGTATG5361861.3019537926913953No Hit
GTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA5031411.221714728114015No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATGC3528470.856774495958083No Hit
ACTTATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTG2640790.6412301992594938RNA PCR Primer, Index 47 (96% over 25bp)
TCGGGGCTGATCCGGGTTGAAGACATTATCTCGTATGCCG2591460.6292520087447346No Hit
TTCGGGGCTGATCCGGGTTGAAGACATATCTCGTATGCCG2575610.6254033503287822No Hit
CGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCTT2400870.5829734089026923RNA PCR Primer, Index 41 (95% over 23bp)
TGTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTTC2256120.5478255662712026RNA PCR Primer, Index 24 (95% over 23bp)
CGGGGCTGATCCGGGTTGAAGACATTGTCATCTCGTATGC2075660.5040067083694504No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCATCTCGTATGCCG1958820.4756359039959564No Hit
TTCGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTC1934300.4696820172855998RNA PCR Primer, Index 41 (95% over 21bp)
TCGGGGCTGATCCGGGTTGAAGACAATCTCGTATGCCGTC1884910.4576892577169001No Hit
GTGAAATTGTTGAAAGGGAAGGGCATCTCGTATGCCGTCT1832260.44490491288409917RNA PCR Primer, Index 34 (95% over 21bp)
CGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATGCC1488380.36140480839970063No Hit
ATTCAATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTG1469760.3568835453268278RNA PCR Primer, Index 47 (96% over 29bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCG1429380.347078572024862No Hit
ATGAGGTAAAGCGAATGATTAGAGGTATCTCGTATGCCGT1400770.34013156147089363No Hit
AGCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCGT1395820.3389296145208012No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1317400.3198878610205496No Hit
AAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCAC1315010.3193075270385858No Hit
TTCGGGGCTGATCCGGGTTGAAGACAATCTCGTATGCCGT1297850.31514077761159887No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTA1264560.30705738085027035No Hit
ACGTGAAATTGTTGAAAGGGAAGGGCATCTCGTATGCCGT1236150.30015893380943703No Hit
TGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCTG1229090.2984446417957699RNA PCR Primer, Index 47 (96% over 25bp)
ATTGTGAAGAGACATAGAGGGTGTAGAATATCTCGTATGC1225790.2976433438290417No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCATCTCGTATGCCG1194550.29005772307734745No Hit
AAATGAGAACTTTGAAGACTGAAGTATCTCGTATGCCGTC1130170.2744251281991761No Hit
GAGCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCG1066450.25895279291435036No Hit
AAATGAGAACTTTGAAGACTATCTCGTATGCCGTCTTCTG767800.1864353269254425Illumina PCR Primer Index 1 (95% over 23bp)
CTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTTCT760580.18468218410126735Illumina PCR Primer Index 1 (95% over 23bp)
TGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCTTCT743910.18063441528146126RNA PCR Primer, Index 2 (96% over 25bp)
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGC735090.17849276435220574No Hit
TCGGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCT724990.1760403069388859No Hit
AAATGAGAACTTTGAAGACTGAATCTCGTATGCCGTCTTC709490.1722766346709198TruSeq Adapter, Index 8 (95% over 22bp)
GTTTTCATGCCAGGTCGTACTGATAACCGCATCTCGTATG674650.16381687068279474No Hit
AGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAA653110.15858658031815026No Hit
TTCGGGGCTGATCCGGGTTGAAGACATTATCTCGTATGCC647590.157246227355623No Hit
AACTTAGAACTGGTACGGACATCTCGTATGCCGTCTTCTG646210.15691113911499116RNA PCR Primer, Index 20 (95% over 24bp)
TGAACTCTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAA643250.1561923991205925Illumina Single End Adapter 1 (96% over 25bp)
TTCGGGGCTGATCCGGGTTGAAGACATTGTATCTCGTATG635350.15427414035175815No Hit
ATTGTGAAGAGACATAGAGGGTGTAGAATCTCGTATGCCG614070.14910698255418922No Hit
TTGTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTT577920.14032912755502963RNA PCR Primer, Index 34 (96% over 25bp)
AGCTGGGACTGAGGACTGCGACGATCTCGTATGCCGTCTT572940.139119896077967Illumina PCR Primer Index 1 (95% over 21bp)
AACGGGGTATTGTAAGCATCTCGTATGCCGTCTTCTGCTT571400.13874595702682713RNA PCR Primer, Index 43 (96% over 27bp)
AATAGTAATTGAACTTAGTACGAGAGGAACATCTCGTATG556680.1351716824600877No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTATCTCGTATG544300.13216560099702834No Hit
AGCTGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCT536950.1303808918893154No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGATCTCGTATGCCGT536210.13020120689071574No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGATCTCGTATGC512910.12454355761048283No Hit
AACTCTGGTGGAGGCTCGTATGCCGTCTTCTGCTTGAAAA512710.1244949940973478TruSeq Adapter, Index 15 (96% over 25bp)
AATGAAGCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAA508840.12355529011818465RNA PCR Primer, Index 47 (96% over 29bp)
GTTGAAAGGGAAGGGCATCTCGTATGCCGTCTTCTGCTTG494600.12009756798296936Illumina PCR Primer Index 3 (96% over 28bp)
TCTGAACTCTGAACTCGTATGCCGTCTTCTGCTTGAAAAA489610.11888590833024995Illumina Single End Adapter 1 (96% over 25bp)
GTGGCGTCGCTGAACCATAGCATCTCGTATGCCGTCTTCT487060.11826672353777812RNA PCR Primer, Index 34 (96% over 27bp)
AAGGATTGGCTCTAAGGGTCGGATCTCGTATGCCGTCTTC487050.11826429536212137TruSeq Adapter, Index 9 (95% over 22bp)
TAGAATAATGTAGATAAGGGAAGTCGGCATCTCGTATGCC480730.11672968834705391No Hit
ATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGT470860.11433307897383937No Hit
AACTGAATGTGGAGACGTATCTCGTATGCCGTCTTCTGCT457150.11100405014843194Illumina PCR Primer Index 2 (96% over 27bp)
TGTGGCGTCGCTGAACCATAGCAGGCATCTCGTATGCCGT454580.1103800090046466No Hit
GGGGCTGATCCGGGTTGAAGACATCTCGTATGCCGTCTTC450010.10927033272951078RNA PCR Primer, Index 41 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC442290.10739578112249802Illumina Paired End PCR Primer 2 (100% over 24bp)
AATGATTAATAGGGACGGTCGGGGGCATCTCGTATGCCGT436210.10591945032319261No Hit
CGGGGCTGATCCGGGTTGAAGACATATCTCGTATGCCGTC432940.1051254368834346No Hit
CTGGGACTGAGGACTGCGACGTATCTCGTATGCCGTCTTC427260.10374623311039928TruSeq Adapter, Index 2 (95% over 24bp)
GCGGAGAATTTGGACAAACTTGGTCATCTCGTATGCCGTC426220.10349370284209705No Hit
AAATGAGAACTTTGAAGACTGATCTCGTATGCCGTCTTCT424730.10313190466924094Illumina Paired End PCR Primer 2 (96% over 25bp)
CGGGGCTGATCCGGGTTGAAGACATTATCTCGTATGCCGT421390.10232089399988568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)