FastQCFastQC Report
Thu 28 Sept 2023
SRR5990809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12435969
Total Bases609.3 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4642223.732897693778426No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2087431.6785422993576131No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1007030.8097720410850172No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA1005420.8084774093598979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA812420.6532824261623682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA560570.45076503487585085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTCCTTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT533530.42902165484651816TruSeq Adapter, Index 1 (100% over 35bp)
CGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCA504800.4059193135653522Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCTGAACTCC492580.39609297835978846Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG490000.3940183511232619No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT430880.34647883088161446Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT417100.33539806990512766No Hit
GCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCTGAACTCCA366030.2943317082890766Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC330110.2654477507944898No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA320510.2577282075888095Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA312510.25129525491740934No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG300260.24144479613932782No Hit
GCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCAG284690.22892466200261516Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC278000.22354510533115673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT272200.21888121464439161No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC271560.21836657843067958Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC267940.215455667346871No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA261340.21014848139296582No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC259110.20835529583581305No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC245770.19762834725625322Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT233410.1876894353789399No Hit
CGTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC229880.18485089501268456TruSeq Adapter, Index 1 (100% over 36bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG222200.1786752604481404No Hit
CTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT213310.17152664179204694TruSeq Adapter, Index 1 (100% over 35bp)
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC199280.16024485104457883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT189540.1524127311671491Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCCGCCGGTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA188730.15176139470916983Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC174810.14056805706093348No Hit
GTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA174090.13998909132050746TruSeq Adapter, Index 1 (100% over 37bp)
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA172700.13887136579385168Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA168650.13561468350395534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT161840.1301386325424259Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG158290.1272840097944921No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC153870.12372980344354349No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGT143150.11510964686386722No Hit
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC141080.11344512036014243TruSeq Adapter, Index 1 (100% over 36bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA138780.11159564646711488Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT138600.11145090503200837No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT132860.10683526149027872No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG131920.1060793895513892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG131110.10542805309340993No Hit
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG128260.1031363137042236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)