FastQCFastQC Report
Thu 28 Sept 2023
SRR5990810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34551086
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG10784213.1212361892184806No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC4118621.1920377842826706No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA2815840.814978724547182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2779680.8045130621943403No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA2695530.7801578219567397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTCCTTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2231490.6458523474486446TruSeq Adapter, Index 1 (100% over 35bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1809930.5238417107931137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCA1514060.4382090913148142Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCTGAACTCC1463590.4236017357023163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT1387140.40147507953874445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1160100.3357636862702376Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCTGAACTCCA1150730.3330517599359974Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1098150.3178337144019149Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG1086960.3145950318319951No Hit
CGTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1026970.29723233590978876TruSeq Adapter, Index 1 (100% over 36bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1005940.291145696549162No Hit
GCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCAG866910.2509067298203015Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT866030.2506520344975553TruSeq Adapter, Index 1 (100% over 35bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC822000.23790858556515418No Hit
GTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA811750.23494196390816774TruSeq Adapter, Index 1 (100% over 37bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC770470.2229944378593483Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC728050.21071696559697137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA721010.2086794030150022Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCCGCCGGTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA692100.2003120828097849Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG671610.1943817337608433No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC668950.19361185926254243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT626750.18139806083085203No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC616390.17839960225852236No Hit
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC599440.17349382303062774TruSeq Adapter, Index 1 (100% over 36bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT584100.16905402047275736No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA574570.16629578589801777Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT544730.15765929904489834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC479070.13865555484999806Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA433710.1255271686684465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG428020.12388033186569014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGGGGGCCCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA417530.12084424784795475Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC406090.1175332086522548Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC405010.11722062802888454Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT380540.11013836149752283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC351230.1016552706910573TruSeq Adapter, Index 1 (100% over 36bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG346380.10025155215092225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)