FastQCFastQC Report
Thu 28 Sept 2023
SRR5990811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40347266
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG13511083.3486977779361804No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC4972041.2323115028413572No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3526550.8740493098094925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT3444470.8537059239652074No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA3254950.8067337201980427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTCCTTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2745120.680373237681086TruSeq Adapter, Index 1 (100% over 35bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2272310.5631880980485766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCA1890720.46861167743063437Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCTGAACTCC1810000.4486053652309428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT1642260.40703129674263433Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1463500.36272594034004685Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCTGAACTCCA1421750.35237827514756515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG1347890.33407220206692567No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1322160.32769506612913996Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1244060.3083381163918269TruSeq Adapter, Index 1 (100% over 36bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1229190.30465261264542687No Hit
GCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCAG1077070.26694993410458095Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1049310.260069666182586TruSeq Adapter, Index 1 (100% over 35bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1000510.24797467070011633No Hit
GTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA971420.2407647645815704TruSeq Adapter, Index 1 (100% over 37bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC938560.23262047049235007Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA879970.21809904046534404Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC870200.2156775628861693Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCGCCGGTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA852600.21131543336790157Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG814080.20176831808132925No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC813600.2016493509126492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT767740.19028302933834476No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC761980.18885542331418442No Hit
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC718360.1780442818603868TruSeq Adapter, Index 1 (100% over 36bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT710080.17599209820065628No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA704230.1745421858323684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT645350.1599488798076182Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC572900.1419922727849763Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG525000.13012034074378176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA519280.12870264865034473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA516750.12807559253209375Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC507360.12574829729479067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC497030.12318802468548923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT472910.11720992445931776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC428510.10620546135641509TruSeq Adapter, Index 1 (100% over 36bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG419900.10407148776821705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA416730.1032858087583927No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT406270.1006933158742404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)