FastQCFastQC Report
Thu 28 Sept 2023
SRR5990815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13162410
Total Bases644.9 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG152802311.608991058628321No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3451472.62221735989078No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2257081.7147923518565371No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT950830.7223829070816059No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA914790.6950019031469161No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT893800.6790549754946092No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG706810.5369913260565504No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC610440.4637752508849064No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC599720.45563084571898305No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA567650.4312660067571212Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG458690.3484848139512445No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC443590.33701275070446823Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG391630.29753669730695215No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT381120.2895518373914807No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA378010.28718904820621755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT341220.2592382398056283TruSeq Adapter, Index 2 (100% over 48bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG327100.24851072106096073No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT272070.20670226804969608Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC261200.19844390199059292No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA251830.1913251448632887Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA226040.1717314686292252No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA225790.1715415338072587No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT203240.15440941286588095No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGT200140.15205422107349642No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACG187690.142595466939565No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT176860.13436749045197652No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT170950.1298774312606886No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC168820.12825918657753405No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTAGATCGGAAGAGCACAC168670.12814522568435416No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACAC167070.1269296428237686No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC165280.1255697094984885Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACG161140.12242438884672337No Hit
AGTGAGTTCAATGGCTGAGGTGAGGTACCCAGATCGGAAGAGCACACGT159610.12126198773628842No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT159050.12083653373508346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA152980.116224916257737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGAGTTCAATGGCTGAGGTGAGGTACCCAGATCGGAAGAGCACACGTC148430.1127681024979468No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGC144440.10973674273936157No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC142170.1080121345559058Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT140860.10701687608880137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)