FastQCFastQC Report
Thu 28 Sept 2023
SRR5990816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28860812
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG340401511.794591919312596No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC7209012.497854183728441No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5351161.8541266267906809No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT2190500.7589876542628115No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1972870.683580905485265No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1775130.6150658546959801No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG1557180.5395482289271695No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1473100.5104152994725166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1244900.4313461450772764No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1139990.39499581647252335No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG1015320.35179883365720965No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC1015150.3517399302556006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA995340.3448759515151549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG850630.29473529712192437No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT804840.27886949265322125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG700710.24278942671467457No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA547780.18980061960834643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT545910.18915268219064663Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC503860.17458275255734315No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA455060.15767401138956175No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC453410.157102301903356Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGT440770.15272266074842247No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA439730.152362310526814No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT428460.14845736149073005No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT421110.14591065559763183No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACG409560.14190868919419175No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT402380.1394208867027026No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC381260.13210300527926933No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT373880.12954590466824012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAGTTCAATGGCTGAGGTGAGGTACCCAGATCGGAAGAGCACACGT363150.12582806055491438No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT356330.12346499467859741No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTAGATCGGAAGAGCACAC352440.1221171462535427No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACG347820.12051635969216666No Hit
GTGAGTTCAATGGCTGAGGTGAGGTACCCAGATCGGAAGAGCACACGTC346480.12005206229124808No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC345580.11974022075331768Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA340640.11802855720067752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACAC332880.1153397901625221No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC301470.10445652048875131No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGATCGGAAGAGCACACGTCTGA295110.10225284028737654Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)