FastQCFastQC Report
Thu 28 Sept 2023
SRR5990817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34838023
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG422179112.118342651074085No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC8812672.52961254431688No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT6594891.8930149968613315No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT2645750.7594432095070378No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2420340.6947409156943263No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA2169090.6226214386505227No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG1913430.5492361033230846No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1766870.5071671259876027Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1536430.4410210074205416No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1400480.4019975530758447No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG1254140.35999172513319716No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC1215230.34882289388235377Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1196840.34354417872678944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG1035880.29734178658760285No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT960350.27566145185678304No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG856040.2457200283724481No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT672540.19304769389468512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA656210.18836028669020627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC617030.17711395391179344No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA551620.15833849125135488No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA543330.15595890731227774No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC534580.15344728373363783Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT528690.15175660226184476No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGT527020.15127724096169293No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT512720.14717253042745854No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACG506240.14531249376579147No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT487470.13992470238624047No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC460800.13226927371854597No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT459130.1317899124183941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAGTTCAATGGCTGAGGTGAGGTACCCAGATCGGAAGAGCACACGT439250.12608350364772422No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT438450.12585386949196284No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTAGATCGGAAGAGCACAC430540.12358336177687235No Hit
GTGAGTTCAATGGCTGAGGTGAGGTACCCAGATCGGAAGAGCACACGTC421190.12089951258141142No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACAC418520.12013310858655785No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA414500.118979196953857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC413600.11872085852862548Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACG410250.11775926550137475No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC372990.10706405469678919No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGATCGGAAGAGCACACGTCTGA363340.10429409269291773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)