FastQCFastQC Report
Thu 28 Sept 2023
SRR5990822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27313593
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG8296413.037465631123668No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT6890072.5225791421875545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA6421152.3508990560121474Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA4661191.7065458945661232Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2478400.9073870288687395No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC2380730.8716282768070828Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1514240.5543906288711266No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1499460.5489794037715946No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC1458180.5338660497723605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1323390.4845169948896873No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT1183290.43322385304635685Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1005000.3679486620453047No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC962640.3524399005286489No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT918560.3363014159286916No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC791180.28966529595721807Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC731280.26773482346317456Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG726440.2659628119962101Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT666030.24384561928560625No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC629550.23048963203046924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAAC556220.203642193833671Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACT555680.2034444900749601Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC525790.19250122091223956No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG520830.1906852752766727No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC511320.18720349241493056No Hit
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA508590.1862039900792254Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG487620.17852649411595173No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC443910.16252347320251861No Hit
GTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT436000.15962747925547546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC430950.1577785829934568No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAA425480.1557759171413296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA407130.14905765052587552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG382030.13986808692653507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT363050.1329191659259183Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT355400.13011836267751373No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC352340.1289980413781519No Hit
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC351800.12880033761944099Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA338800.12404080268751166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGCACCGCACGTTCGTAGATCGGAAGAGCACACGTCTG338330.12386872719381883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA334430.12244086671424005Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT329320.12057000336792015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG326650.11959246811651619No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT323530.11845017973285317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC321060.1175458680957866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG319900.1171211711326298Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA318680.11667450708517184TruSeq Adapter, Index 3 (100% over 36bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCA316860.11600817219470175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCT306040.1120467746590498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG304270.11139874567216404No Hit
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG292190.10697603936618665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT288620.10566899785026453Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCCCGTCACGCACCGCACGTTCGTGAGATCGGAAGAGCACACGTCT286460.10487818281542088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTGA281490.10305857599913713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGT278630.10201147831411267No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG276410.10119869619496782No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT274440.10047744359374469Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)