FastQCFastQC Report
Thu 28 Sept 2023
SRR5990823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32400447
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG10206633.1501509840280906No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT8596782.6532905549111714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA7694922.3749425432309623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA5513421.7016493630473677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2963630.9146879979773118No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC2774110.8561949778038557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1831300.5652082516022079No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1785690.5511312853183785No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC1723770.5320204378661814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1599450.4936505968575063No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT1407720.4344754873289248Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1213700.37459359742783793No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC1154480.35631607181221914No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1105520.3412051691755981No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC942460.2908787030006098Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG869010.26820926266850575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC859680.2653296727665516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT809290.24977741819426133No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC739400.2282067281355717Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAAC658010.20308670432849277Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACT654520.20200955869528592Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC638470.1970559233334034No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG636480.19644173427607342No Hit
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA606010.18703754303142792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC605650.18692643345321747No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG588570.18165490124256617No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC560440.17297292225628863No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC524160.1617755458744134No Hit
GTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT514390.15876015537686872Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAA510900.15768300974366187Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA489600.15110902636621032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG461320.14238075172234507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT436840.13482530040403456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT430420.13284384625928156No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC424840.1311216477970196No Hit
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC415540.128251317026583Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCCCGTCACGCACCGCACGTTCGTAGATCGGAAGAGCACACGTCTG406790.1255507370006346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA406160.1253562952387663Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA405850.12526061754641843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT391790.1209211712418659Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG389080.12008476302811501No Hit
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT386920.11941810555885232Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC377810.11660641595469345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG377320.11645518347324037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA377240.11643049245586025TruSeq Adapter, Index 3 (100% over 36bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCA375840.11599839965170851Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG374480.11557865235624681No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCT372580.11499224069346946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTGA348000.1074059256034338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGT346390.10690901887865929No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGAGATCGGAAGAGCACACGTCT344520.10633186634739947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT340280.10502324242625419Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG338290.1044090533689242Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAGATCGGAAGAGCACACGTC331720.10238130356658351No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG331470.1023041441372707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)