FastQCFastQC Report
Thu 28 Sept 2023
SRR5990827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15088082
Total Bases739.3 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG12130068.039497664448007No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT3789662.511691015465054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2867421.9004536163045773No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2685991.780206390712882No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA2265761.5016885512684781No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1820991.2069062190939843No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1512901.002711941782925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1012860.6712980483536609No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC937440.621311575586612No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA800260.5303921333407388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC773850.5128882518003283Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT602770.3995007450251132No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC580420.3846877290301047Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC580170.38452203533888535No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA547540.3628956947609378Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA533790.3537825417438744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG488730.3239179108385015No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA414560.2747599065275494Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC378650.2509596647208041No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG374850.24844112061427026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC369540.2449217866127716No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG329130.21813905836407832No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG316230.20958926389716068No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT315290.208966255618176No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA310310.20566563728908685Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC304210.20162271122333508No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC281340.18646505235059035No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT273310.18114297098862533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG264490.1752972975624072No Hit
CCTCGCCCGTCACGCACCGCACGTTCAGATCGGAAGAGCACACGTCTGA262950.1742766244244961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCT257430.17061810772237318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA257010.1703397423211247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT253070.167728409747508No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG252710.16748981083215217No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG249610.1654352090610324No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCT244620.1621279629842945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC233360.15466511913177566No Hit
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC229080.15182844313810065No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC226520.15013173974001467Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA225140.14921711056448395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC224950.14909118335915725Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT224950.14909118335915725No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC223120.1478783055394317No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG219700.14561161584355123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCGAAGTGGAGAAGGGTTCCATGTGAGATCGGAAGAGCACACGTCT204840.13576278283747398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT198990.13188555046294154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC194650.1290091079833739No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT183850.12185114052269866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT183270.1214667311590698No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACG183160.12139382593493327No Hit
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGA181610.12036652504937341Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT181040.11998874343339332No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT178710.11844447823122914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG176920.11725811140209869Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT175820.11652905916073361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA174540.11568070746169064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATAGATCGGAAGAGCAC166500.11035199835207682No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT165060.10939760269065345Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAA161300.10690556957471467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC158990.10537455986784801Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGA158360.10495701176597529Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGCACCGCACGTTCGTAGATCGGAAGAGCACACGTCTG152720.1012189620920671Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCT152460.10104664065319899Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC151510.10041700462656554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)