FastQCFastQC Report
Thu 28 Sept 2023
SRR5990828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28506876
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG25225468.848903682044991No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5873102.0602397821494014No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT5784012.0289876730091363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5311541.863248712345751No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3783321.3271605068194776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA3549021.2449698100907303No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3410131.1962482314793106No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2028100.7114423902499875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1781990.625108833391635No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1640660.5755313209346404No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC1566260.5494323544958065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1525510.5351375576895904Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1090830.3826550478558226No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA1014170.3557632902321531Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT998820.3503786244413453No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG966230.338946294921969No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA881860.3093499266633075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA812070.28486811392451417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG717930.2518445023579574Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC705530.24749467461815175No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG638680.22404419200476405No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT611820.21462190385224955No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA574380.20148823041851374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC565210.19827146264641557No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT538980.18907017380648794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG530460.1860814211981699No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC528710.1854675342187618No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC520270.1825068450152167Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC515770.18092827849673881No Hit
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA511420.17940233086221025Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCT495930.1739685541130498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG486320.1705974376147004No Hit
GCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCT482140.1691311247153143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG478810.16796298549164068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG466240.16355352301669251No Hit
CCTCGCCCGTCACGCACCGCACGTTCAGATCGGAAGAGCACACGTCTGA461180.1617785126648041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT449480.15767423971676167No Hit
AGCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA438710.15389620384920466Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG437270.15339106256329174No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC425090.14911840918661168Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC423730.14864133130547172No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT422470.1481993326802979No Hit
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC416560.146126148652697No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT411190.14424239260731342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCGAAGTGGAGAAGGGTTCCATGTGAGATCGGAAGAGCACACGTCT407710.14302163449969055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC407310.14288131747582583No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT401020.14067483227555344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT390080.13683716167285395No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT384940.1350340879161926No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC382570.1342027095497942No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTC357940.12556268880532542No Hit
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT348600.1222862862980847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA345850.12132160675901492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT338320.11868013878476195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGA337570.11841704436501566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA332930.11678936688818516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAA332660.11669465289707648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG315420.11064698916850799Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG306260.10743372932200637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCAGATCGGAAGAGCACACGT304980.10698471484563934No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACG303890.10660235095560804No Hit
CTCGCCCGTCACGCACCGCACGTTCGTAGATCGGAAGAGCACACGTCTG294700.10337856733231661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC293460.10294358455833602Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)