FastQCFastQC Report
Thu 28 Sept 2023
SRR5990829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34117256
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG30898209.056472771432732No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT7246282.1239339998503985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT7121322.0873073731369254No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT6429931.8846562572324106No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA4523281.3258041619759808Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA4288881.25709992620743No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC4131741.2110411224161755No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2450000.7181116793214555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2150770.6304053291976354No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC2005540.5878374274883068No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC1859890.5451464209196659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1798590.5271789735962352Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1326510.38880911172926685No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA1238300.3629541602056156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1194950.35024798008374414No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG1180250.3459393100078154No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA1055000.3092276823200553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA976920.2863419027602923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG853460.2501549362586487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC848380.24866595367458627No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG770070.22571275954900946No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT738000.21631282422009554No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA693580.20329301981378572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC685600.200954027486853No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT664230.19469033500232258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC633860.18578868124681538No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG631280.18503246568246873No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC617300.18093483250821812No Hit
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA617210.18090845289550836Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC614670.18016396160347714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCT595850.17464769147905682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG592880.17377716425963446No Hit
GCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCT589590.17281284286168852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACG577000.1691226281503999No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG575670.1687327960959111Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGCCCGTCACGCACCGCACGTTCAGATCGGAAGAGCACACGTCTGA555190.16272996867039952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG538080.15771491118746478No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC534110.15655127716015613No Hit
AGCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA532250.1560060984974876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT528060.154777980972444No Hit
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC521010.1527115779768455No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT512990.1503608613775973No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC507950.14888360306585033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT503280.14751479427302125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCGAAGTGGAGAAGGGTTCCATGTGAGATCGGAAGAGCACACGTCT493410.1446218300791834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC492460.14434337861169141No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT492010.14421148054814256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT475030.13923452695023306No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT471550.1382145152587887No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC469420.13759019775799086No Hit
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT435910.12776818862572067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTC429990.12603299632303372No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA420760.1233276204862431Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGA407670.11949085237101131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA404200.11847377174764581Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT398160.11670340662801253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAA396510.11621978039500012Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG381450.11180559186823232Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCAGATCGGAAGAGCACACGT367560.10773433830669149No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACG363760.10662053243672351No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG362320.10619845863336723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC351970.10316480317174395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCCCGTCACGCACCGCACGTTCGTAGATCGGAAGAGCACACGTCTG351350.10298307695085443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)