FastQCFastQC Report
Thu 28 Sept 2023
SRR5990833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10204205
Total Bases500 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4577454.485846766112598No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA2234162.189450329545516No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1794021.7581183443492168No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT997200.9772441851178019No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC612050.599801748396862No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT448680.43970108401389424No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC444260.4353695363823051No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC407760.3995999688363768No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC374810.3673093592298469No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG352530.34547522320455143No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA306790.30065056513466754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC297670.2917130731889451Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTG279750.27415168550612223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG221100.21667538039465106No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC194740.19084289270942714No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT188730.18495316391624825No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA179130.17554527765759312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA171960.16851876260816007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA168270.1649026063274895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCT166670.1633346252843803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCT162420.15916967563862153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC154570.15147676864586707No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT149180.146194632506893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT148750.1457732376015574No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT147320.14437185454427856No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTG139950.13714934186445685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT138860.13608115477883873No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG137950.13518936556057037No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG134960.13225920098626007No Hit
GGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACT130810.1281922501556956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTC122260.1198133514565809No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC122200.11975455216746429Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC122160.11971535264138657Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG114890.11259083877675918No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT114390.11210084470078757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG113240.11097385832605283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCACACGTCTGAA111500.10926867894167158Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC111130.1089060833254526Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC110240.10803389387022311No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT108550.10637771389343904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT106570.1044373373525914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)