FastQCFastQC Report
Thu 28 Sept 2023
SRR5990834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27562736
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG12595244.5696624602144No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA5634262.0441584609017043No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5167531.8748247634052004No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2594600.9413434138033321No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1573100.5707343421930247No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT1257550.45625006167747645No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1081300.39230503096644687No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1065870.3867068929586671No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC986370.3578636025102878No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA947010.34358345267320345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG931360.33790549675474885No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC839620.30462142800337383Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA554010.2009996395132907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC552430.200426401791172No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG547200.198528912369222No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA467460.16959854783647024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA467060.16945342436251612Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC454640.16494734049624102No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT453270.1644502925979482No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCT440510.15982085377881208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT415650.15080142987256417No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT396160.14373028860415019No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT391800.14214844273805038No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTG387780.1406899518248116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC385880.14000061532352956Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG380100.13790358112489268No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG370910.13456936931079702No Hit
GGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACT354110.1284741834047244Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG346160.12558985435988648No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT345780.12545198705963007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTC337710.12252412097260591No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG324140.11760080711871275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT323660.11742665894996782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT303990.11029021211827446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC300230.10892605146310584Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTC298260.10821131835388184No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC291910.10590748320486035Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCACACGTCTGAA289390.10499320531894947Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT282340.10243540409050828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGA281450.1021125043609604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA281290.10205445497137874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT276020.10014245320203335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)