FastQCFastQC Report
Thu 28 Sept 2023
SRR5990835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32967851
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG15582494.726571349767384No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA6815972.06745959874667No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT6311711.9145045274561572No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3177000.9636660879109166No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1925670.5841054062031523No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT1511780.4585618880648302No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1317600.3996620829182952No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1297900.39368656452615No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1203630.3650920407277987No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG1149970.3488155779398542No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1135510.3444294867748583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC997970.3027100553202573Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG671540.20369541223660587No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA667770.20255187394531723Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC666390.20213328433206032No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA567550.17215256159705405Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA560750.1700899461114405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT558840.16951059382062847No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC553500.1678908340128084No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCT515630.15640388571278122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT496480.15059519651432543No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT469910.14253582983009722No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG464980.14104043360302737No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT460320.1396269353437687No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC458930.13920531247244475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTG453400.1375279207613502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG450800.13673927366390973No Hit
GGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACT424130.12864957439901073Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT421520.12785789404350317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG418990.12709047975253224No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT410330.12446367826644206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTC409020.12406632145965475No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG386920.11736282113141072Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT383620.11636184596927474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTG373050.1131556921923725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC362650.1100011038026106Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTC359280.10897889583400507No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC346930.10523282212116282Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCACACGTCTGAA346030.10495982889512573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA341800.10367676073275144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT337900.10249379008659071No Hit
GGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGA336370.10202970160232767Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT333040.10101962666599046Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)