FastQCFastQC Report
Thu 28 Sept 2023
SRR5990839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11484798
Total Bases562.7 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG7369476.416717124672109No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2760082.403246448043753No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1751341.524920159675425No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1572891.3695408486940737No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1141990.9943492258200798No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT1089110.9483057516553622No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1050960.9150879275369058No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA913850.7957040254430248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC871860.7591426510070095No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG854310.743861581196291No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA848410.7387243554479583No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT769680.6701728667757151No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA701340.6106681197179088Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA566620.4933652294102169Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGC540530.47064824300784397No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACG492130.4285055775469451No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT486360.42348154490832146No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC475030.4136163300390656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG381080.33181254036858115No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG361840.31505996013164533No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC356360.3102884351992956Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC354810.30893882504507264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCAC337300.2936925838834954No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA335430.2920643445361425No Hit
AGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG322050.28041416139839814No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGT302720.2635832167008945No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACG296980.2585853055491268No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACAC239730.2087368014657289No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT237600.20688217589895794No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACAC208520.18156174797327737No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT195700.17039916592351037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG195300.17005087943209798No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACAC194740.16956327834412063No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA193950.16887541252358118No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACA193460.168448761571601No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACG188620.16423449502551113No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT186300.16221443337531927Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA184930.16102155214223185No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC183120.1594455557685908No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC181040.15763446601324638No Hit
CGAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACAC178840.15571889031047825No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC176770.15391650771741913No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCA170750.14867479602166273No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT170040.14805658749940573Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGT166250.14475657299327338No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC162420.14142172983799975No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACG161950.1410124932105902No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT158850.1383132729021442No Hit
AGGGGAGATACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTC158450.13796498641073182No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT155060.13501325839601183No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT152860.13309768269324374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTC148770.12953645331855204No Hit
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTG145050.12629738894841686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC144170.1255311586673096No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCAC143830.12523511514960908No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTC143400.12486070717134076No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTC143140.1246343209519227No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCA142010.12365041161368272No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACGT136430.11879181505847992No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA135280.11779049139566931Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACG130760.11385485404270934No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTC129860.11307120943703146No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCT129800.11301896646331959Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACA125870.1095970516851929No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACG121150.10548727108652674No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGAGATCGGAAGAGCACACG120090.10456431188428389No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC119740.10425956120429807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC119630.10416378241915965No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC119100.10370230281803824No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAAGATCGGAAGAGCACAC116760.10166482684327578No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA115090.10021073074162906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA115020.1001497806056319Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)