FastQCFastQC Report
Thu 28 Sept 2023
SRR5990840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23920886
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG18524497.744065165479238No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC6855272.8658094018758336No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT4490201.877104384845946No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA3206361.3404018563526452No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2615881.0935548123092096No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2277300.952013232285794No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT2258340.9440871044659466No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA2130020.8904436064784557No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG2058320.8604698003242857No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA2053500.8584548247920247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1813150.7579777772445385No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1575040.6584371498614223No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1351700.565071042937122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA1287940.5384165118298712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGC1058020.44229967067273346No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC962640.4024265656380788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACG947490.39609318818709305No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT943070.3942454305413269No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC821620.34347389975438203Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG776270.3245155718730485No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG757280.31657690271171396No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC699200.2922968655926875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA675990.2825940477288341No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCAC664590.27782833796373596No Hit
AGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG607900.2541293830002785No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACG601130.25129922027135615No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGT581020.2428923410278365No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG550310.23005418779220801No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT487500.2037967991653821No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA485420.2029272661556098No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACAC482990.20191141749515465No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACAC449810.18804069381042157No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA424450.17743907980665932No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC412020.17224278398383738No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACAC408760.1708799582089058No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACG407890.1705162593057799No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACA403880.1688398999936708No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT371240.15519492045570552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC367240.1535227415907588No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC358920.15004460955166962No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGT354610.14824283682468953No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC348490.14568440316132103No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCA348340.14562169645388554No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTC348220.14557153108793713No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC339440.14190109847937907No Hit
CGAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACAC338350.14144542973868107No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT336850.14081836266432607No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT333260.13931758213303638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACGT329790.1378669669676951No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT329640.1378042602602596Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTC324860.13580600651664826No Hit
AGGGGAGATACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTC321290.13431358687968328No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT321100.13423415838359834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACG320420.13394988797655738No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC314580.13150850683373516No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACG312130.1304842972789553No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCAC305510.12771684125746846No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCA305160.12757052560678564No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC300870.12577711377413028No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT284360.11887519550906267No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTC277950.11619552887798554No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT267400.11178515712168854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC264750.11067733862366136Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACA253920.1061499143468181No Hit
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC249590.10433978072551327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA248350.10382140527737976No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAAGATCGGAAGAGCACAC245870.10278465438111281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)