FastQCFastQC Report
Thu 28 Sept 2023
SRR5990841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28998587
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG23113187.9704504222912655No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC8323912.8704536534831853No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5523261.9046652169638472No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA3904171.3463311160643792No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC3210321.1070608371366508No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2804930.9672643705019145No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGT2750750.948580701535561No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA2604250.898060998627278No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACG2538810.8754943818469499No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA2512470.8664111806551127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2231930.7696685359186639No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1932270.6663324664750045No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1638000.5648551082851037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA1513030.5217599050602018Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGC1348120.4648916169605092No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC1162170.4007678029277771Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACG1151870.39721590572671694No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGT1135500.3915708030877504No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC977090.33694400351299875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACG957550.330205744162638No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG907360.3128980043062098No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC846410.2918797388300333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA807620.278503225001963No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCAC803580.27711005367261515No Hit
AGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG743870.25651939523811973No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACG741490.2556986655936029No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGT699380.24117726839587048No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAG684380.23600460256908382No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACAC594910.2051513751342436No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA592460.20430650638253514No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT588880.20307196347187534No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACAC550900.18997477359845152No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA521910.17997773477721518No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACAC501410.1729084248139401No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACG500380.17255323509383405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC498460.17189113386800536No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACA491450.16947377470495373No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT460010.15863186713200889Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC444870.1534109230908389No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCA439190.151452206964429No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGT435350.1501280045127716No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC431230.14870724563234755No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT428210.14766581557922115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTC424900.14652438065344356No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC418080.14417254192419787No Hit
CGAAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACAC415690.14334836383579655No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT414870.14306559143726555No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC408860.14099307666266636No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT403170.13903091209237195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACGT400360.13806189936082058No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTC398390.13738255591556925No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACG397430.13705150530265492No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT387050.13347202055051854Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC385060.13278578021749818No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACG382070.1317546954960254No Hit
AGGGGAGATACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTC379040.13070981699901446No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCA373440.1287786884236808No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCAC370560.12778553658493774No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC363110.12521644589096703No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT361000.12448882423133237No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTC343090.11831266123414909No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT333690.11507112398269613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACA314720.10852942593375324No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC314080.10830872552514369Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC305320.10528788868230028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA303770.10475337988019899No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAAGATCGGAAGAGCACAC303220.10456371546655015No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTC294320.10149460040932339No Hit
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA290740.10026005749866364Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGAGATCGGAAGAGCACACG290410.10014625885047433No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)