FastQCFastQC Report
Thu 28 Sept 2023
SRR5990843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33198222
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT10426363.1406380739305857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTC4749761.4307272238856648No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG4175361.2577059096719096No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT3975091.1973803898293107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA3761251.132967301682602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3430211.0332511180869868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC3303240.9950050939475011No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3299820.9939749182953231No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT3203890.9650787924726811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA2313440.6968565967177398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2246350.6766476831198972No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG2142330.6453146798042377No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA2052360.6182138308491341Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG1802170.5428513611361475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC1784890.5376462631040902No Hit
CGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTG1577700.47523629428106123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA1530660.4610668607493497Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCT1398240.4211791824272999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG1289800.38851478250853316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1283240.3865387730704373No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1222350.3681974293683559No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA1154610.34779272215240925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA1142150.3440395091038309Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACG1120090.3373945749263319No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1074120.32354744781211475No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG995430.2998443711834929No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTG936310.28203618856455626Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC895970.26988493540407077No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCTG879690.2649810583229427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC829360.24982060786267407No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTGA814860.24545290407420015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG808370.24349798010266938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC808320.24348291905512287No Hit
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTG774180.23319923579039867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT710450.21400242458767824No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT709440.2136981914272397No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC688950.20752617414269958No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCT670120.201854183636702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG657900.19817326361634668No Hit
CTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACACGTCT628990.18946496592498238Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG594270.17900657450871918Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT585920.17649137956846No Hit
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTG581870.17527143471719658Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCT550200.1657317672012676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC548310.16516245960401132No Hit
GCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGA546170.16451784676902276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG545840.16441844385521612No Hit
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG533340.16065318196860062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTGA526550.15860789171179107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCT524700.158050632952572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC521700.15714697009978426No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC502290.15130027144224772Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCT489070.14731813047096318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG486790.1466313467028445No Hit
CAGTGCGGTAACGCGACCGATCCCGGAAGATCGGAAGAGCACACGTCTG477070.1437034790598123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA462910.13943819039465427Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA425970.12831108846732817Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT421380.12692848430256295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTG417960.12589830865038495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC413090.12443136261935955No Hit
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA410740.12372349338467584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGCACCGCACGTTCGTAGATCGGAAGAGCACACGTCTG400820.1207353815514578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCT399580.12036186757230552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT397870.11984677974621652Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTC393740.11860273721887876No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCAGATCGGAAGAGCACACGT383860.11562667422369789No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAA378250.11393682468898486Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGTGAGATCGGAAGAGCACACGT373990.11265362343802629No Hit
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA371080.1117770704708222Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCATCTAAGGCTAAATACCGGCACGAGATCGGAAGAGCACACGTCTG364060.10966249939529894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT356180.10728887830197653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGTGTTGACGCGATGTGATTTCAGATCGGAAGAGCACACGTCTGA356000.10723465853080927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG350650.10562312644333784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTC348070.1048459763899404No Hit
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA347990.10482187871386606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGT346630.1044122182206023No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC335300.10099938484657402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)