FastQCFastQC Report
Thu 28 Sept 2023
SRR5990845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35635389
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT22741096.381602849908556Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT7182702.0156086972980707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA6789611.905299813059428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA5085041.4269635165200527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA4482631.2579152706877987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG4177091.1721746604197305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT4015751.1268994425737853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA3563250.999918928905196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG3019240.8472588863839818No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA2871110.8056906576774004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTC2661160.7467745055343721No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC2539070.7125136195370282No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGA2354140.6606185777851338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC2300620.6455997996822764No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2272660.6377536667271964No Hit
AGCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA2198510.6169456996807303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC2121340.5952902604767413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCTG1839240.516127381126666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC1637650.4595572115124098No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1590240.44625302111897813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTGA1581510.44380320921991334Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACT1569980.4405676615456618Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTG1547630.43429580634015247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1505210.4223919093460717No Hit
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA1472810.41329982394748094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCT1423740.3995298044873314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG1409390.39550290863949883No Hit
GGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTGAA1348470.37840754313079056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCT1333490.3742038567335409Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG1216910.34148918649379695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTG1154610.3240065654958895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCTCGGATAGCCGGTCAGATCGGAAGAGCACACGTCTGA1106390.31047507296749305Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1061240.2978050835926051No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG924640.25947240256027515No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC914200.256542730598507No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCT897220.25177780436183816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCT873180.2450317014920196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAAC841290.23608273225248083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG838510.23530260887568816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTC818370.229650923692737No Hit
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA751660.2109307688489103Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACACGTCT743590.2086661660968539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGCTACACTGACTGGCTCAGCGAGATCGGAAGAGCACACGTCTGA742490.20835748418517333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAAC737620.20699086517618764Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT724300.2032530078456559No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC714510.20050573883169903No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTC698990.1961505176778062No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT685710.19242388514406283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAGGGCAGGGCGCCCTGGAATAGATCGGAAGAGCACACGTCTGAAC669800.18795922222148326Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTGA661050.18550379792402436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT652270.18303995502897413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA651450.18280984669481226Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGA650100.18243100980320434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC648170.18188941335816483No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTGA643250.18050876335319366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT633530.17778113773361642No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC633210.17769133935930936No Hit
AGTGCGGTAACGCGACCGATCCCAGATCGGAAGAGCACACGTCTGAACT621160.1743098693268088Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA608940.17088069390795763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTAACGCGACCGATCCCGGAAGATCGGAAGAGCACACGTCTG592890.1663767441966187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTGA592060.16614382966325975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC590200.16562187661259992No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCAGATCGGAAGAGCACACGT581850.16327870028302482No Hit
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA579140.16251822030061183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA577760.16213096481141262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA573550.16094955494943525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTGA559540.1570180698743039Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC558020.15659152759634531Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT556240.15609202413926224No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC544320.15274703469632395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCTG537340.150788307656751Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG532830.14952271181886073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT530030.1487369760436739No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGA529490.14858544128703072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG523100.14679228000008643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT519520.1457876606875261No Hit
GCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCT513680.1441488403564221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA504080.14145488912721002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTG496120.1392211545663217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCTG483900.13579197914747052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTG482770.13547487863819868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG481130.13501466196987497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTG469840.13184646307635367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCCGGGCCGTACCCATATCCGCAGCAGATCGGAAGAGCACACGTCT469040.13162196714058602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAGGGCAGGGCGCCCTGGAATGGGTAGATCGGAAGAGCACACGTCT459090.12882979893947558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGA458500.12866423318684692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTC456050.12797671438355843No Hit
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG455960.12795145859078458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG454770.12761752088633016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTG443280.12439319800886697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTG439440.12331561751718215Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA434440.1219125179186342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGA423470.11883411739942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTG408810.11472022937647741Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCGCCGGTGAAATACCACTAGATCGGAAGAGCACACGTCTGAACTC401100.11255664979551647Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCCGCGCTCTCCCCCCTCCCGGCGCAGATCGGAAGAGCACACGTCT387410.10871496309469218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCTG387370.1087037382979038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCT381340.10701160018205498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCAGATCGGAAGAGCACACGTCTG379410.10647000373701547Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTAACGCGACCGATCCCGGAAGATCGGAAGAGCACACGTCTGA376760.10572636094978505Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCATCTAAGGCTAAATACCGGCACGAGATCGGAAGAGCACACGTCTG373450.10479750901554633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGAAGAGCACACGTCT365980.10270127821531568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAA365450.10255254965786958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTG360910.10127853522238807Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGGGCCGTACCCATATCCGCAGCAGATCGGAAGAGCACACGTCTG359300.10082673715165562Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTG356550.10005503237245424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)