FastQCFastQC Report
Thu 28 Sept 2023
SRR5990847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28995301
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT11414453.93665511525471Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA4993421.7221480128797422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA4441091.531658526324662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTC4361561.5042299440174807No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA4167821.437412220690518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT3320481.1451786618804198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA3165011.091559628920562Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT2959451.020665382987402Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC2433360.8392256386646926Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA2350640.8106968780906948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG2343880.8083654658387578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA2127200.7336361157278554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2034640.7017137018167185Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTGA1721180.5936065295545647Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC1289280.4446513591978231Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAAC1288470.4443720035877538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCTG1236620.42648979570862183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTG1213550.41853333407368315Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC1165250.40187546251028744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACT1132120.390449473174981Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC1047020.36109988994423614No Hit
CTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTGA1040010.35868225682499383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT1004900.34657339822062894Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGA959510.33091913755266755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA907830.3130955598633034Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTG871640.30061422711217933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGA864330.29809312895217055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCTG782070.26972301477401456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGTGTTGACGCGATGTGATTTAGATCGGAAGAGCACACGTCTGAA750190.25872812977523496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA748940.2582970254387082Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC709130.24456721452900246Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCGCCGGTGAAATACCACTAGATCGGAAGAGCACACGTCTGAACTC691570.23851106080947393Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCT645780.222718846753824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCT638510.2202115439325841Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA621190.2142381622456687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCC611360.2108479577432219Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGAGAAGACGGTCGAACTTGAGATCGGAAGAGCACACGTCTGAACT598380.20637137031272756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT585760.20201894093115294No Hit
GCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA580000.20003241214843742Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCT566680.1954385643384078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAA539850.18618534085919647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT533180.18388496811948943Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAAC523450.18052925196396477Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTG520610.17954978291137588Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACT517700.17854617201594147Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG514120.1773114891961287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGA507180.1749179979197319Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAA496910.17137604469082765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAA494650.1705966080503872Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG485750.16752714517431636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTG482210.16630625769327242Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA476440.16431628007586468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAAC474760.16373687584757268Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAA467100.16109506847333643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACT466300.1608191616979593Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCTCGGATAGCCGGTCAGATCGGAAGAGCACACGTCTGA461630.15920855589669514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTGA444460.15328690673016293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGTGTTGACGCGATGTGATTTCAGATCGGAAGAGCACACGTCTGA424430.14637889084165742Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGA424130.14627542580089098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA420140.14489934075869743Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG419590.14470965485062562No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG406740.14027790227113007Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC406200.1400916651977505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGAA404730.13958468649799496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCA396430.136722153703457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC391000.13484943646558453No Hit
CTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC387330.13358371413354186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACG382820.13202828968735314No Hit
CAGTGCGGTAACGCGACCGATCCCGGAAGATCGGAAGAGCACACGTCTG378960.13069703949615835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCT378930.13068669299208172Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGAGATCGGAAGAGCACACGTCTG377580.13022110030863276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT372990.12863808518490635No Hit
CGTGGCGCAATGAAGGTGAAGGCCAGATCGGAAGAGCACACGTCTGAAC369380.12739305586101693Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCTGGTATTGCAGTACTTCCAGGAGATCGGAAGAGCACACGTCTGAA365110.12592040344744138Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGAAC362920.12516510864984642Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGGTGTTGACGCGATGTGATTAGATCGGAAGAGCACACGTCTGAAC354690.12232671769815391Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCTGA351200.12112307439057109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGAA348560.12021258203182647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAGATCGGAAGAGCACACGTCTGA347510.11985045438914395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAAC341780.1178742721105051Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCAGATCGGAAGAGCACACGTCTG338660.11679823568653416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTG335360.11566012023810342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTGA328550.11331146381270538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC326780.11270102007218342No Hit
GGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAACT321040.11072138895885233Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG318730.1099247081449508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA317930.10964880136957364Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTGAA315850.10893144375359304Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTGA313040.10796232120508077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC304090.10487561415554886Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA301500.10398236597026532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT297970.10276492732391362Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT297490.10259938325868734No Hit
GGGCCGCCGGTGAAATACCACTACTAGATCGGAAGAGCACACGTCTGAA295830.10202687669977972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGGGACATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGTCTGA294590.10159922119794514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph