FastQCFastQC Report
Thu 28 Sept 2023
SRR5990849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5990849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29762561
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT16864225.666252981388261Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTC8014252.6927286264108794No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA6342352.1309826126857834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT5411561.8182440684455885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT4833461.6240067513007366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3808431.2796042652377932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTC3570811.1997657056460969No Hit
CGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTG3241391.0890830261549065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2996311.0067379618306367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG2635210.8854110370407977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGA2577980.8661821810293812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2241880.753255071026986No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA2204620.7407359870677795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG1966880.6608571083651034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG1844450.6197215353880333No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1832560.615726583475125No Hit
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACG1705760.5731227228732098No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTG1612690.5418518923825136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGA1597760.5368355229914522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1554200.52219968570581No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTGA1314950.44181345818997225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCT1308950.43979750264098577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAAC1225400.4117253216213484Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACACGTCT1165830.3917102429458271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1100260.3696792087213194Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1069570.3593675960882533No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1007700.33857973445228723No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC994680.3342051109109865No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC957460.32169946665544No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCT930100.3125067093520615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG904620.3039456181206987No Hit
AAGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAA902080.30309219693829437Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTCTG825730.27743916257744083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC798260.26820944608899755No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTC778300.26150303396270236No Hit
AACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAA770060.25873445500876086Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA762450.2561775513874629Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGCGCTACACTGACTGGCTCAGCGTGAGATCGGAAGAGCACACGTCT696010.2338542036083521Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA669180.22483952237846738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCT663140.2228101271258209Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC660260.2218424684623074No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCT615600.20683703932601769Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCT607560.20413565889037572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCTG588130.19760732283757437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC584750.19647166787831197No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA576400.1936661297393057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG570030.191525856931465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGTGAGATCGGAAGAGCACACGT566770.1904305210831823No Hit
CAAGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGA565920.19014492738040925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT564830.1897786954556767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC564140.18954686056754322No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCT561400.18862624086683938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACGT555880.1867715617617718No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCACACGTCTG548460.18427849673285843Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTGA539380.18122768400205883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGCTACACTGACTGGCTCAGCGTGAGATCGGAAGAGCACACGTCTG503060.1690244330788604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTACCATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTG501160.16838604715501465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA501160.16838604715501465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTG498820.1675998244909099Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG494980.16630961293955854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCT491720.16521427709127584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTG480860.16156539754761023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAAGAAATTCAATGAAGCGCGGGTAGATCGGAAGAGCACACGTCTGA479050.1609572509569993Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACAC477270.1603591841441333No Hit
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA475240.15967711918339286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGT468510.15741588904261297No Hit
CGCGCGCTACACTGACTGGCTCAGCGAGATCGGAAGAGCACACGTCTGA459940.1545364325334772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCTGA456660.15343437683336458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTC447450.15033988506567025No Hit
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACT441950.14849192581243262Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT441340.14828697033161897No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGAGATCGGAAGAGCACACGTCTG435910.14646253055978617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGT431570.14500432271268593No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC426020.1431395638298734No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC422840.14207110738891052No Hit
CCAGTGCGGTAACGCGACCGATCCCGGAGAGATCGGAAGAGCACACGTC401240.134813667412559No Hit
CCAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGT397470.13354697534261248No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA393260.13213244653240694Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGGTGTTGACGCGATGTGATTTCAGATCGGAAGAGCACACGTCTGA391690.13160493816375546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC391420.13151422016405107No Hit
GCTACCATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGA389580.13089599379569522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGACATGTGGCGTACGGAAGACCCGAGATCGGAAGAGCACACGTCT389430.13084559490697054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCT375380.1261248989964271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAA363270.12205602871338928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCCCGTCACGCACCGCACGTTCGAGATCGGAAGAGCACACGTCTG362990.1219619507877699Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA362920.12193843130636507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT361830.12157219938163251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGA357280.12004343309031773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGTGTTGACGCGATGTGATTTCTAGATCGGAAGAGCACACGTCTG355250.11936136812957729Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCT353970.11893129761246016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA349850.11754700813548942Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCTGA346860.11654239028691113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTC343020.11525217873555975No Hit
CAGTGCGGTAACGCGACCGATCCCGGAAGATCGGAAGAGCACACGTCTG341620.1147817891074629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGA340400.11437187814583563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCTCTTTCTCGATTCCGTGGGTGGAGATCGGAAGAGCACACGTCTGA335940.11287335118775564Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTGA328230.11028284830730797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT324720.10910351431115085No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG322830.10846848831322009No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC320810.10778978327839461No Hit
GGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGA319560.10736979253902243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGTGTTGACGCGATGTGATTTCTGAGATCGGAAGAGCACACGTCT318950.1071648370582088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT318290.10694308194782029Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAAC317100.10654325076393796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATGGGCGAGCGCCGTTCCGAAGGGACGAGATCGGAAGAGCACACGTC314810.1057738277294081No Hit
AGCTCTTTCTCGATTCCGTGGGTGGAGATCGGAAGAGCACACGTCTGAA312670.10505480358360292Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTTTCTCGATTCCGTGGGTGGTGAGATCGGAAGAGCACACGTCTG307500.1033177218855595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGCGAGCGCCGTTCCGAAGGGACGGGCAGATCGGAAGAGCACACGT307410.10328748255232473No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCT306560.10300188884955162Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG306410.10295148996082697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAACT301250.10121776818869854Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGA300620.10100609285605495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGAAGGCCGAAGTGGAGAAGGGTAGATCGGAAGAGCACACGTCT300300.10089857522677567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA299370.10058610211668277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT298970.10045170508008368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCGCCGGTGAAATACCACTACTAGATCGGAAGAGCACACGTCTGAA297680.10001827463705155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)